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000009906 084__ $$2WoS$$aBiochemistry & Molecular Biology
000009906 1001_ $$0P:(DE-Juel1)VDB2662$$aBecker, J. S.$$b0$$uFZJ
000009906 245__ $$aBioimaging of metals in brain tissue by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) and metallomics
000009906 260__ $$aCambridge$$bRSC Publ.$$c2010
000009906 300__ $$a104 - 111
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000009906 440_0 $$020981$$aMetallomics$$v2$$x1756-5901$$y2
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000009906 520__ $$aLaser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has been developed and established as an emerging technique in the generation of quantitative images of metal distributions in thin tissue sections of brain samples (such as human, rat and mouse brain), with applications in research related to neurodegenerative disorders. A new analytical protocol is described which includes sample preparation by cryo-cutting of thin tissue sections and matrix-matched laboratory standards, mass spectrometric measurements, data acquisition, and quantitative analysis. Specific examples of the bioimaging of metal distributions in normal rodent brains are provided. Differences to the normal were assessed in a Parkinson's disease and a stroke brain model. Furthermore, changes during normal aging were studied. Powerful analytical techniques are also required for the determination and characterization of metal-containing proteins within a large pool of proteins, e.g., after denaturing or non-denaturing electrophoretic separation of proteins in one-dimensional and two-dimensional gels. LA-ICP-MS can be employed to detect metalloproteins in protein bands or spots separated after gel electrophoresis. MALDI-MS can then be used to identify specific metal-containing proteins in these bands or spots. The combination of these techniques is described in the second section.
000009906 536__ $$0G:(DE-Juel1)FUEK409$$2G:(DE-HGF)$$aFunktion und Dysfunktion des Nervensystems (FUEK409)$$cFUEK409$$x0
000009906 536__ $$0G:(DE-HGF)POF2-89571$$a89571 - Connectivity and Activity (POF2-89571)$$cPOF2-89571$$fPOF II T$$x1
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000009906 650_2 $$2MeSH$$aAnimals
000009906 650_2 $$2MeSH$$aBrain Chemistry
000009906 650_2 $$2MeSH$$aDisease Models, Animal
000009906 650_2 $$2MeSH$$aHistocytochemistry
000009906 650_2 $$2MeSH$$aHumans
000009906 650_2 $$2MeSH$$aLasers
000009906 650_2 $$2MeSH$$aMass Spectrometry: methods
000009906 650_2 $$2MeSH$$aMetalloproteins: analysis
000009906 650_2 $$2MeSH$$aMetalloproteins: metabolism
000009906 650_2 $$2MeSH$$aMetals, Heavy: analysis
000009906 650_2 $$2MeSH$$aMetals, Heavy: chemistry
000009906 650_2 $$2MeSH$$aMetals, Heavy: metabolism
000009906 650_2 $$2MeSH$$aMice
000009906 650_2 $$2MeSH$$aParkinson Disease: metabolism
000009906 650_2 $$2MeSH$$aPlasma Gases: chemistry
000009906 650_2 $$2MeSH$$aRats
000009906 650_2 $$2MeSH$$aStroke: metabolism
000009906 650_7 $$00$$2NLM Chemicals$$aMetalloproteins
000009906 650_7 $$00$$2NLM Chemicals$$aMetals, Heavy
000009906 650_7 $$00$$2NLM Chemicals$$aPlasma Gases
000009906 650_7 $$2WoSType$$aJ
000009906 7001_ $$0P:(DE-Juel1)138474$$aMatusch, A.$$b1$$uFZJ
000009906 7001_ $$0P:(DE-Juel1)VDB1883$$aPalm, C.$$b2$$uFZJ
000009906 7001_ $$0P:(DE-Juel1)VDB64651$$aSalber, D.$$b3$$uFZJ
000009906 7001_ $$0P:(DE-HGF)0$$aMorton, K.A.$$b4
000009906 7001_ $$0P:(DE-HGF)0$$aBecker, S.$$b5
000009906 773__ $$0PERI:(DE-600)2474317-3$$a10.1039/b916722f$$gVol. 2, p. 104 - 111$$p104 - 111$$q2<104 - 111$$tMetallomics$$v2$$x1756-5901$$y2010
000009906 8567_ $$uhttp://dx.doi.org/10.1039/b916722f
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000009906 9132_ $$0G:(DE-HGF)POF3-571$$1G:(DE-HGF)POF3-570$$2G:(DE-HGF)POF3-500$$aDE-HGF$$bKey Technologies$$lDecoding the Human Brain$$vConnectivity and Activity$$x0
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000009906 9201_ $$0I:(DE-Juel1)INM-4-20090406$$gINM$$kINM-4$$lPhysik der Medizinischen Bildgebung$$x2
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