TY  - JOUR
AU  - Becker, J. S.
AU  - Matusch, A.
AU  - Palm, C.
AU  - Salber, D.
AU  - Morton, K.A.
AU  - Becker, S.
TI  - Bioimaging of metals in brain tissue by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) and metallomics
JO  - Metallomics
VL  - 2
SN  - 1756-5901
CY  - Cambridge
PB  - RSC Publ.
M1  - PreJuSER-9906
SP  - 104 - 111
PY  - 2010
N1  - Record converted from VDB: 12.11.2012
AB  - Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has been developed and established as an emerging technique in the generation of quantitative images of metal distributions in thin tissue sections of brain samples (such as human, rat and mouse brain), with applications in research related to neurodegenerative disorders. A new analytical protocol is described which includes sample preparation by cryo-cutting of thin tissue sections and matrix-matched laboratory standards, mass spectrometric measurements, data acquisition, and quantitative analysis. Specific examples of the bioimaging of metal distributions in normal rodent brains are provided. Differences to the normal were assessed in a Parkinson's disease and a stroke brain model. Furthermore, changes during normal aging were studied. Powerful analytical techniques are also required for the determination and characterization of metal-containing proteins within a large pool of proteins, e.g., after denaturing or non-denaturing electrophoretic separation of proteins in one-dimensional and two-dimensional gels. LA-ICP-MS can be employed to detect metalloproteins in protein bands or spots separated after gel electrophoresis. MALDI-MS can then be used to identify specific metal-containing proteins in these bands or spots. The combination of these techniques is described in the second section.
KW  - Animals
KW  - Brain Chemistry
KW  - Disease Models, Animal
KW  - Histocytochemistry
KW  - Humans
KW  - Lasers
KW  - Mass Spectrometry: methods
KW  - Metalloproteins: analysis
KW  - Metalloproteins: metabolism
KW  - Metals, Heavy: analysis
KW  - Metals, Heavy: chemistry
KW  - Metals, Heavy: metabolism
KW  - Mice
KW  - Parkinson Disease: metabolism
KW  - Plasma Gases: chemistry
KW  - Rats
KW  - Stroke: metabolism
KW  - Metalloproteins (NLM Chemicals)
KW  - Metals, Heavy (NLM Chemicals)
KW  - Plasma Gases (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:21069140
UR  - <Go to ISI:>//WOS:000274397000002
DO  - DOI:10.1039/b916722f
UR  - https://juser.fz-juelich.de/record/9906
ER  -