Home > Publications database > Bioimaging of metals in brain tissue by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) and metallomics > print

001     9906
005     20210129210510.0
024 7 _ |2 pmid
|a pmid:21069140
024 7 _ |2 DOI
|a 10.1039/b916722f
024 7 _ |2 WOS
|a WOS:000274397000002
024 7 _ |a altmetric:21804061
|2 altmetric
037 _ _ |a PreJuSER-9906
041 _ _ |a eng
082 _ _ |a 690
084 _ _ |2 WoS
|a Biochemistry & Molecular Biology
100 1 _ |a Becker, J. S.
|b 0
|u FZJ
|0 P:(DE-Juel1)VDB2662
245 _ _ |a Bioimaging of metals in brain tissue by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) and metallomics
260 _ _ |a Cambridge
|b RSC Publ.
|c 2010
300 _ _ |a 104 - 111
336 7 _ |a Journal Article
|0 PUB:(DE-HGF)16
|2 PUB:(DE-HGF)
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|0 0
|2 EndNote
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a article
|2 DRIVER
440 _ 0 |a Metallomics
|x 1756-5901
|0 20981
|y 2
|v 2
500 _ _ |a Record converted from VDB: 12.11.2012
520 _ _ |a Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has been developed and established as an emerging technique in the generation of quantitative images of metal distributions in thin tissue sections of brain samples (such as human, rat and mouse brain), with applications in research related to neurodegenerative disorders. A new analytical protocol is described which includes sample preparation by cryo-cutting of thin tissue sections and matrix-matched laboratory standards, mass spectrometric measurements, data acquisition, and quantitative analysis. Specific examples of the bioimaging of metal distributions in normal rodent brains are provided. Differences to the normal were assessed in a Parkinson's disease and a stroke brain model. Furthermore, changes during normal aging were studied. Powerful analytical techniques are also required for the determination and characterization of metal-containing proteins within a large pool of proteins, e.g., after denaturing or non-denaturing electrophoretic separation of proteins in one-dimensional and two-dimensional gels. LA-ICP-MS can be employed to detect metalloproteins in protein bands or spots separated after gel electrophoresis. MALDI-MS can then be used to identify specific metal-containing proteins in these bands or spots. The combination of these techniques is described in the second section.
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588 _ _ |a Dataset connected to Web of Science, Pubmed
650 _ 2 |2 MeSH
|a Animals
650 _ 2 |2 MeSH
|a Brain Chemistry
650 _ 2 |2 MeSH
|a Disease Models, Animal
650 _ 2 |2 MeSH
|a Histocytochemistry
650 _ 2 |2 MeSH
|a Humans
650 _ 2 |2 MeSH
|a Lasers
650 _ 2 |2 MeSH
|a Mass Spectrometry: methods
650 _ 2 |2 MeSH
|a Metalloproteins: analysis
650 _ 2 |2 MeSH
|a Metalloproteins: metabolism
650 _ 2 |2 MeSH
|a Metals, Heavy: analysis
650 _ 2 |2 MeSH
|a Metals, Heavy: chemistry
650 _ 2 |2 MeSH
|a Metals, Heavy: metabolism
650 _ 2 |2 MeSH
|a Mice
650 _ 2 |2 MeSH
|a Parkinson Disease: metabolism
650 _ 2 |2 MeSH
|a Plasma Gases: chemistry
650 _ 2 |2 MeSH
|a Rats
650 _ 2 |2 MeSH
|a Stroke: metabolism
650 _ 7 |0 0
|2 NLM Chemicals
|a Metalloproteins
650 _ 7 |0 0
|2 NLM Chemicals
|a Metals, Heavy
650 _ 7 |0 0
|2 NLM Chemicals
|a Plasma Gases
650 _ 7 |a J
|2 WoSType
700 1 _ |a Matusch, A.
|b 1
|u FZJ
|0 P:(DE-Juel1)138474
700 1 _ |a Palm, C.
|b 2
|u FZJ
|0 P:(DE-Juel1)VDB1883
700 1 _ |a Salber, D.
|b 3
|u FZJ
|0 P:(DE-Juel1)VDB64651
700 1 _ |a Morton, K.A.
|b 4
|0 P:(DE-HGF)0
700 1 _ |a Becker, S.
|b 5
|0 P:(DE-HGF)0
773 _ _ |a 10.1039/b916722f
|g Vol. 2, p. 104 - 111
|p 104 - 111
|q 2<104 - 111
|0 PERI:(DE-600)2474317-3
|t Metallomics
|v 2
|y 2010
|x 1756-5901
856 7 _ |u http://dx.doi.org/10.1039/b916722f
909 C O |o oai:juser.fz-juelich.de:9906
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913 2 _ |a DE-HGF
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|l Decoding the Human Brain
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|b Programmungebundene Forschung
|l ohne Programm
914 1 _ |y 2010
915 _ _ |0 StatID:(DE-HGF)0010
|a JCR/ISI refereed
920 1 _ |k INM-2
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|l Zentralabteilung für Chemische Analysen
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