Journal Article

PreJuSER-10029

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Characterization of the 5-HT(1A) receptor of the honeybee (Apis mellifera) and involvement of serotonin in phototactic behavior

Thamm, M.FZJ* ; Balfanz, S. ; Scheiner, R. ; Baumann, A. ; Blenau, W.FZJ*

2010
Birkhäuser Basel

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Please use a persistent id in citations: doi:10.1007/s00018-010-0350-6

Abstract: Serotonin plays a key role in modulating various physiological and behavioral processes in both protostomes and deuterostomes. The vast majority of serotonin receptors belong to the superfamily of G-protein-coupled receptors. We report the cloning of a cDNA from the honeybee (Am5-ht1A) sharing high similarity with members of the 5-HT(1) receptor class. Activation of Am5-HT(1A) by serotonin inhibited the production of cAMP in a dose-dependent manner (EC(50) = 16.9 nM). Am5-HT(1A) was highly expressed in brain regions known to be involved in visual information processing. Using in vivo pharmacology, we could demonstrate that Am5-HT(1A) receptor ligands had a strong impact on the phototactic behavior of individual bees. The data presented here mark the first comprehensive study-from gene to behavior-of a 5-HT(1A) receptor in the honeybee, paving the way for the eventual elucidation of additional roles of this receptor subtype in the physiology and behavior of this social insect.

Keyword(s): Amino Acid Sequence (MeSH) ; Animals (MeSH) ; Bees: metabolism (MeSH) ; Bees: physiology (MeSH) ; Behavior, Animal: drug effects (MeSH) ; Brain: metabolism (MeSH) ; Humans (MeSH) ; Immunohistochemistry (MeSH) ; Insect Proteins: chemistry (MeSH) ; Insect Proteins: genetics (MeSH) ; Insect Proteins: immunology (MeSH) ; Insect Proteins: metabolism (MeSH) ; Ligands (MeSH) ; Light (MeSH) ; Molecular Sequence Data (MeSH) ; Movement: drug effects (MeSH) ; Mushroom Bodies: metabolism (MeSH) ; Optic Lobe, Nonmammalian: metabolism (MeSH) ; Prazosin: pharmacology (MeSH) ; Receptor, Serotonin, 5-HT1A: chemistry (MeSH) ; Receptor, Serotonin, 5-HT1A: genetics (MeSH) ; Receptor, Serotonin, 5-HT1A: immunology (MeSH) ; Receptor, Serotonin, 5-HT1A: metabolism (MeSH) ; Serotonin: analogs & derivatives (MeSH) ; Serotonin: metabolism (MeSH) ; Serotonin: pharmacology (MeSH) ; Serotonin Antagonists: pharmacology (MeSH) ; Serotonin Receptor Agonists: pharmacology (MeSH) ; Insect Proteins ; Ligands ; Serotonin Antagonists ; Serotonin Receptor Agonists ; Receptor, Serotonin, 5-HT1A ; Prazosin ; Serotonin ; 5-carboxamidotryptamine ; J ; 5-HT (auto) ; Biogenic amine (auto) ; Cyclic AMP (auto) ; GPCR (auto) ; Phototaxis (auto) ; Signal transduction (auto)

Note: We thank J. Schlenstedt for initiating the work on the honeybee 5-HT<INF>1A</INF> receptor and J. Erber for supporting the phototaxis experiments. This work was supported by the German Science Foundation (Research Training Group 837 Functional Insect Science, scholarship to M.T.).

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Contributing Institute(s):

1. Zelluläre Biophysik (ISB-1)

Research Program(s):

1. BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung (P45)

Appears in the scientific report 2010

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