%0 Journal Article
%A Figueroa Miranda, Gabriela
%A Liang, Yuanying
%A Suranglikar, Mohit
%A Stadler, Matthias
%A Samane, Nagesh
%A Tintelott, Marcel
%A Lo, Young
%A Tanner, Julian A.
%A Vu, Duy Tam
%A Knoch, Joachim
%A Ingebrandt, Sven
%A Offenhäusser, Andreas
%A Pachauri, Vivek
%A Mayer, Dirk
%T Delineating charge and capacitance transduction in system-integrated graphene-based BioFETs used as aptasensors for malaria detection
%J Biosensors and bioelectronics
%V 208
%@ 0956-5663
%C Amsterdam [u.a.]
%I Elsevier Science
%M FZJ-2023-01333
%P 114219
%D 2022
%X Despite significant eradication efforts, malaria remains a persistent infectious disease with high mortality due to the lack of efficient point-of-care (PoC) screening solutions required to manage low-density asymptomatic parasitemia. In response, we demonstrate a quantitative electrical biosensor based on system-integrated two-dimensional field-effect transistors (2DBioFETs) of reduced graphene oxide (rGO) as transducer for high sensitivity screening of the main malaria biomarker, Plasmodium falciparum lactate dehydrogenase (PfLDH). The 2DBioFETs were biofunctionalized with pyrene-modified 2008s aptamers as specific PfLDH receptors. While we systematically optimize biosensor interface for optimal performance, aptamer-protein transduction at 2DBioFETs is elucidated based on delineation of charge and capacitance in an updated analytical model for two-dimensional rGO/biofunctional layer/electrolyte (2DiBLE) interfaces. Our 2DBioFET-aptasensors display a limit-of-detection down to 0.78 fM (0.11 pg/mL), dynamic ranges over 9 orders of magnitude (subfemto to submicromolar), high sensitivity, and selectivity in human serum validating their diagnostic potential as rapid PoC tests for malarial management.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ 35367704
%U <Go to ISI:>//WOS:000792492900006
%R 10.1016/j.bios.2022.114219
%U https://juser.fz-juelich.de/record/1005145