Journal Article

FZJ-2023-01333

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Delineating charge and capacitance transduction in system-integrated graphene-based BioFETs used as aptasensors for malaria detection

Figueroa Miranda, G.FZJ* ; Liang, Y. ; Suranglikar, M. ; Stadler, M. ; Samane, N. ; Tintelott, M. ; Lo, Y. ; Tanner, J. A. ; Vu, D. T. ; Knoch, J. ; Ingebrandt, S. ; Offenhäusser, A.FZJ* ; Pachauri, V. ; Mayer, D. (Corresponding author)FZJ*

2022
Elsevier Science Amsterdam [u.a.]

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Please use a persistent id in citations: http://hdl.handle.net/2128/34137  doi:10.1016/j.bios.2022.114219

Abstract: Despite significant eradication efforts, malaria remains a persistent infectious disease with high mortality due to the lack of efficient point-of-care (PoC) screening solutions required to manage low-density asymptomatic parasitemia. In response, we demonstrate a quantitative electrical biosensor based on system-integrated two-dimensional field-effect transistors (2DBioFETs) of reduced graphene oxide (rGO) as transducer for high sensitivity screening of the main malaria biomarker, Plasmodium falciparum lactate dehydrogenase (PfLDH). The 2DBioFETs were biofunctionalized with pyrene-modified 2008s aptamers as specific PfLDH receptors. While we systematically optimize biosensor interface for optimal performance, aptamer-protein transduction at 2DBioFETs is elucidated based on delineation of charge and capacitance in an updated analytical model for two-dimensional rGO/biofunctional layer/electrolyte (2DiBLE) interfaces. Our 2DBioFET-aptasensors display a limit-of-detection down to 0.78 fM (0.11 pg/mL), dynamic ranges over 9 orders of magnitude (subfemto to submicromolar), high sensitivity, and selectivity in human serum validating their diagnostic potential as rapid PoC tests for malarial management.

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Contributing Institute(s):

1. Bioelektronik (IBI-3)

Research Program(s):

1. 5241 - Molecular Information Processing in Cellular Systems (POF4-524) (POF4-524)

Appears in the scientific report 2023

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Database coverage:
; ; ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Agriculture, Biology and Environmental Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 10 ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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