TY  - JOUR
AU  - Figueroa Miranda, Gabriela
AU  - Liang, Yuanying
AU  - Suranglikar, Mohit
AU  - Stadler, Matthias
AU  - Samane, Nagesh
AU  - Tintelott, Marcel
AU  - Lo, Young
AU  - Tanner, Julian A.
AU  - Vu, Duy Tam
AU  - Knoch, Joachim
AU  - Ingebrandt, Sven
AU  - Offenhäusser, Andreas
AU  - Pachauri, Vivek
AU  - Mayer, Dirk
TI  - Delineating charge and capacitance transduction in system-integrated graphene-based BioFETs used as aptasensors for malaria detection
JO  - Biosensors and bioelectronics
VL  - 208
SN  - 0956-5663
CY  - Amsterdam [u.a.]
PB  - Elsevier Science
M1  - FZJ-2023-01333
SP  - 114219
PY  - 2022
AB  - Despite significant eradication efforts, malaria remains a persistent infectious disease with high mortality due to the lack of efficient point-of-care (PoC) screening solutions required to manage low-density asymptomatic parasitemia. In response, we demonstrate a quantitative electrical biosensor based on system-integrated two-dimensional field-effect transistors (2DBioFETs) of reduced graphene oxide (rGO) as transducer for high sensitivity screening of the main malaria biomarker, Plasmodium falciparum lactate dehydrogenase (PfLDH). The 2DBioFETs were biofunctionalized with pyrene-modified 2008s aptamers as specific PfLDH receptors. While we systematically optimize biosensor interface for optimal performance, aptamer-protein transduction at 2DBioFETs is elucidated based on delineation of charge and capacitance in an updated analytical model for two-dimensional rGO/biofunctional layer/electrolyte (2DiBLE) interfaces. Our 2DBioFET-aptasensors display a limit-of-detection down to 0.78 fM (0.11 pg/mL), dynamic ranges over 9 orders of magnitude (subfemto to submicromolar), high sensitivity, and selectivity in human serum validating their diagnostic potential as rapid PoC tests for malarial management.
LB  - PUB:(DE-HGF)16
C6  - 35367704
UR  - <Go to ISI:>//WOS:000792492900006
DO  - DOI:10.1016/j.bios.2022.114219
UR  - https://juser.fz-juelich.de/record/1005145
ER  -