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@ARTICLE{FigueroaMiranda:1005145,
author = {Figueroa Miranda, Gabriela and Liang, Yuanying and
Suranglikar, Mohit and Stadler, Matthias and Samane, Nagesh
and Tintelott, Marcel and Lo, Young and Tanner, Julian A.
and Vu, Duy Tam and Knoch, Joachim and Ingebrandt, Sven and
Offenhäusser, Andreas and Pachauri, Vivek and Mayer, Dirk},
title = {{D}elineating charge and capacitance transduction in
system-integrated graphene-based {B}io{FET}s used as
aptasensors for malaria detection},
journal = {Biosensors and bioelectronics},
volume = {208},
issn = {0956-5663},
address = {Amsterdam [u.a.]},
publisher = {Elsevier Science},
reportid = {FZJ-2023-01333},
pages = {114219},
year = {2022},
abstract = {Despite significant eradication efforts, malaria remains a
persistent infectious disease with high mortality due to the
lack of efficient point-of-care (PoC) screening solutions
required to manage low-density asymptomatic parasitemia. In
response, we demonstrate a quantitative electrical biosensor
based on system-integrated two-dimensional field-effect
transistors (2DBioFETs) of reduced graphene oxide (rGO) as
transducer for high sensitivity screening of the main
malaria biomarker, Plasmodium falciparum lactate
dehydrogenase (PfLDH). The 2DBioFETs were biofunctionalized
with pyrene-modified 2008s aptamers as specific PfLDH
receptors. While we systematically optimize biosensor
interface for optimal performance, aptamer-protein
transduction at 2DBioFETs is elucidated based on delineation
of charge and capacitance in an updated analytical model for
two-dimensional rGO/biofunctional layer/electrolyte (2DiBLE)
interfaces. Our 2DBioFET-aptasensors display a
limit-of-detection down to 0.78 fM (0.11 pg/mL), dynamic
ranges over 9 orders of magnitude (subfemto to
submicromolar), high sensitivity, and selectivity in human
serum validating their diagnostic potential as rapid PoC
tests for malarial management.},
cin = {IBI-3},
ddc = {610},
cid = {I:(DE-Juel1)IBI-3-20200312},
pnm = {5241 - Molecular Information Processing in Cellular Systems
(POF4-524)},
pid = {G:(DE-HGF)POF4-5241},
typ = {PUB:(DE-HGF)16},
pubmed = {35367704},
UT = {WOS:000792492900006},
doi = {10.1016/j.bios.2022.114219},
url = {https://juser.fz-juelich.de/record/1005145},
}