TY  - JOUR
AU  - Weiß, Lennart JK
AU  - Rinklin, Philipp
AU  - Thakur, Bhawana
AU  - Music, Emir
AU  - Url, Heike
AU  - Kopic, Inola
AU  - Hoven, Darius
AU  - Banzet, Marko
AU  - von Trotha, Tassilo
AU  - Mayer, Dirk
AU  - Wolfrum, Bernhard
TI  - Prototype Digital Lateral Flow Sensor Using Impact Electrochemistry in a Competitive Binding Assay
JO  - ACS sensors
VL  - 7
IS  - 7
SN  - 2379-3694
CY  - Washington, DC
PB  - ACS Publications
M1  - FZJ-2023-01335
SP  - 1967-1976
PY  - 2022
AB  - This work demonstrates a lateral flow assay concept on the basis of stochastic-impact electrochemistry. To this end, we first elucidate requirements to employ silver nanoparticles as redox-active labels. Then, we present a prototype that utilizes nanoimpacts from biotinylated silver nanoparticles as readouts to detect free biotin in solution based on competitive binding. The detection is performed in a membrane-based microfluidic system, where free biotin and biotinylated particles compete for streptavidin immobilized on embedded latex beads. Excess nanoparticles are then registered downstream at an array of detection electrodes. In this way, we establish a proof of concept that serves as a blueprint for future “digital” lateral flow sensors.
LB  - PUB:(DE-HGF)16
C6  - 35801574
UR  - <Go to ISI:>//WOS:000828468100001
DO  - DOI:10.1021/acssensors.2c00728
UR  - https://juser.fz-juelich.de/record/1005147
ER  -