Journal Article

FZJ-2023-01335

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Prototype Digital Lateral Flow Sensor Using Impact Electrochemistry in a Competitive Binding Assay

Weiß, L. J. ; Rinklin, P. ; Thakur, B. ; Music, E. ; Url, H. ; Kopic, I. ; Hoven, D. ; Banzet, M. ; von Trotha, T. ; Mayer, D. (Corresponding author)FZJ* ; Wolfrum, B.

2022
ACS Publications Washington, DC

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Please use a persistent id in citations: doi:10.1021/acssensors.2c00728

Abstract: This work demonstrates a lateral flow assay concept on the basis of stochastic-impact electrochemistry. To this end, we first elucidate requirements to employ silver nanoparticles as redox-active labels. Then, we present a prototype that utilizes nanoimpacts from biotinylated silver nanoparticles as readouts to detect free biotin in solution based on competitive binding. The detection is performed in a membrane-based microfluidic system, where free biotin and biotinylated particles compete for streptavidin immobilized on embedded latex beads. Excess nanoparticles are then registered downstream at an array of detection electrodes. In this way, we establish a proof of concept that serves as a blueprint for future “digital” lateral flow sensors.

Keyword(s): Chemistry (2nd)

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Contributing Institute(s):

1. Bioelektronik (IBI-3)

Research Program(s):

1. 5241 - Molecular Information Processing in Cellular Systems (POF4-524) (POF4-524)

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Database coverage:
; Clarivate Analytics Master Journal List ; Current Contents - Physical, Chemical and Earth Sciences ; Essential Science Indicators ; IF >= 5 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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