%0 Journal Article
%A Martinez Roque, Mateo Alejandro
%A Franco-Urquijo, Pablo Alberto
%A García-Velásquez, Víctor Miguel
%A Choukeife, Moujab
%A Mayer, Günther
%A Molina, Sergio
%A Figueroa Miranda, Gabriela
%A Mayer, Dirk
%A Alvarez-Salas, Luis M
%T DNA aptamer selection for SARS-CoV-2 spike glycoprotein detection
%J Analytical biochemistry
%V 645
%@ 0003-2697
%C San Diego, Calif.
%I Elsevier
%M FZJ-2023-01338
%P 114633
%D 2022
%X The rapid spread of SARS-CoV-2 infection throughout the world led to a global public health and economic crisis triggering an urgent need for the development of low-cost vaccines, therapies and high-throughput detection assays. In this work, we used a combination of Ideal-Filter Capillary Electrophoresis SELEX (IFCE-SELEX), Next Generation Sequencing (NGS) and binding assays to isolate and validate single-stranded DNA aptamers that can specifically recognize the SARS-CoV-2 Spike glycoprotein. Two selected non-competing DNA aptamers, C7 and C9 were successfully used as sensitive and specific biological recognition elements for the development of electrochemical and fluorescent aptasensors for the SARS-CoV-2 Spike glycoprotein with detection limits of 0.07 fM and 41.87 nM, respectively.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ 35247355
%U <Go to ISI:>//WOS:000793071600006
%R 10.1016/j.ab.2022.114633
%U https://juser.fz-juelich.de/record/1005150