Journal Article

FZJ-2023-01338

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png DNA aptamer selection for SARS-CoV-2 spike glycoprotein detection

Martinez Roque, M. A.FZJ* ; Franco-Urquijo, P. A. ; García-Velásquez, V. M. ; Choukeife, M. ; Mayer, G. ; Molina, S. ; Figueroa Miranda, G.FZJ* ; Mayer, D. (Corresponding author)FZJ* ; Alvarez-Salas, L. M.

2022
Elsevier San Diego, Calif.

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Please use a persistent id in citations: http://hdl.handle.net/2128/34139  doi:10.1016/j.ab.2022.114633

Abstract: The rapid spread of SARS-CoV-2 infection throughout the world led to a global public health and economic crisis triggering an urgent need for the development of low-cost vaccines, therapies and high-throughput detection assays. In this work, we used a combination of Ideal-Filter Capillary Electrophoresis SELEX (IFCE-SELEX), Next Generation Sequencing (NGS) and binding assays to isolate and validate single-stranded DNA aptamers that can specifically recognize the SARS-CoV-2 Spike glycoprotein. Two selected non-competing DNA aptamers, C7 and C9 were successfully used as sensitive and specific biological recognition elements for the development of electrochemical and fluorescent aptasensors for the SARS-CoV-2 Spike glycoprotein with detection limits of 0.07 fM and 41.87 nM, respectively.

Keyword(s): Biology (2nd) ; Chemistry (2nd)

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Contributing Institute(s):

1. Bioelektronik (IBI-3)

Research Program(s):

1. 5241 - Molecular Information Processing in Cellular Systems (POF4-524) (POF4-524)

Appears in the scientific report 2023

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Database coverage:
; ; ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF < 5 ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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