TY  - JOUR
AU  - Martinez Roque, Mateo Alejandro
AU  - Franco-Urquijo, Pablo Alberto
AU  - García-Velásquez, Víctor Miguel
AU  - Choukeife, Moujab
AU  - Mayer, Günther
AU  - Molina, Sergio
AU  - Figueroa Miranda, Gabriela
AU  - Mayer, Dirk
AU  - Alvarez-Salas, Luis M
TI  - DNA aptamer selection for SARS-CoV-2 spike glycoprotein detection
JO  - Analytical biochemistry
VL  - 645
SN  - 0003-2697
CY  - San Diego, Calif.
PB  - Elsevier
M1  - FZJ-2023-01338
SP  - 114633
PY  - 2022
AB  - The rapid spread of SARS-CoV-2 infection throughout the world led to a global public health and economic crisis triggering an urgent need for the development of low-cost vaccines, therapies and high-throughput detection assays. In this work, we used a combination of Ideal-Filter Capillary Electrophoresis SELEX (IFCE-SELEX), Next Generation Sequencing (NGS) and binding assays to isolate and validate single-stranded DNA aptamers that can specifically recognize the SARS-CoV-2 Spike glycoprotein. Two selected non-competing DNA aptamers, C7 and C9 were successfully used as sensitive and specific biological recognition elements for the development of electrochemical and fluorescent aptasensors for the SARS-CoV-2 Spike glycoprotein with detection limits of 0.07 fM and 41.87 nM, respectively.
LB  - PUB:(DE-HGF)16
C6  - 35247355
UR  - <Go to ISI:>//WOS:000793071600006
DO  - DOI:10.1016/j.ab.2022.114633
UR  - https://juser.fz-juelich.de/record/1005150
ER  -