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| 001 | 1005150 | ||
| 005 | 20240226075353.0 | ||
| 024 | 7 | _ | |2 doi |a 10.1016/j.ab.2022.114633 |
| 024 | 7 | _ | |2 Handle |a 2128/34139 |
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| 037 | _ | _ | |a FZJ-2023-01338 |
| 082 | _ | _ | |a 540 |
| 100 | 1 | _ | |0 P:(DE-Juel1)190451 |a Martinez Roque, Mateo Alejandro |b 0 |
| 245 | _ | _ | |a DNA aptamer selection for SARS-CoV-2 spike glycoprotein detection |
| 260 | _ | _ | |a San Diego, Calif. |b Elsevier |c 2022 |
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| 520 | _ | _ | |a The rapid spread of SARS-CoV-2 infection throughout the world led to a global public health and economic crisis triggering an urgent need for the development of low-cost vaccines, therapies and high-throughput detection assays. In this work, we used a combination of Ideal-Filter Capillary Electrophoresis SELEX (IFCE-SELEX), Next Generation Sequencing (NGS) and binding assays to isolate and validate single-stranded DNA aptamers that can specifically recognize the SARS-CoV-2 Spike glycoprotein. Two selected non-competing DNA aptamers, C7 and C9 were successfully used as sensitive and specific biological recognition elements for the development of electrochemical and fluorescent aptasensors for the SARS-CoV-2 Spike glycoprotein with detection limits of 0.07 fM and 41.87 nM, respectively. |
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| 700 | 1 | _ | |0 P:(DE-HGF)0 |a Franco-Urquijo, Pablo Alberto |b 1 |
| 700 | 1 | _ | |0 P:(DE-HGF)0 |a García-Velásquez, Víctor Miguel |b 2 |
| 700 | 1 | _ | |0 P:(DE-HGF)0 |a Choukeife, Moujab |b 3 |
| 700 | 1 | _ | |0 P:(DE-HGF)0 |a Mayer, Günther |b 4 |
| 700 | 1 | _ | |0 P:(DE-Juel1)188110 |a Molina, Sergio |b 5 |
| 700 | 1 | _ | |0 P:(DE-Juel1)171348 |a Figueroa Miranda, Gabriela |b 6 |
| 700 | 1 | _ | |0 P:(DE-Juel1)128707 |a Mayer, Dirk |b 7 |e Corresponding author |
| 700 | 1 | _ | |0 P:(DE-HGF)0 |a Alvarez-Salas, Luis M |b 8 |
| 773 | _ | _ | |0 PERI:(DE-600)1461105-3 |a 10.1016/j.ab.2022.114633 |p 114633 |t Analytical biochemistry |v 645 |x 0003-2697 |y 2022 |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/1005150/files/Martinez_2022_COVID-19%20aptamer.pdf |y Published on 2022-03-02. Available in OpenAccess from 2024-03-02. |
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