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@ARTICLE{Sharma:1005578,
author = {Sharma, Vikas and Hünnefeld, Max and Luthe, Tom and
Frunzke, Julia},
title = {{S}ystematic analysis of prophage elements in
actinobacterial genomes reveals a remarkable phylogenetic
diversity},
journal = {Scientific reports},
volume = {13},
number = {1},
issn = {2045-2322},
address = {[London]},
publisher = {Macmillan Publishers Limited, part of Springer Nature},
reportid = {FZJ-2023-01539},
pages = {4410},
year = {2023},
note = {IBT-1},
abstract = {Actinobacteria represent one of the largest bacterial phyla
harboring many species of high medical, biotechnological and
ecological relevance. Prophage elements are major
contributors to bacterial genome diversity and were shown to
significantly shape bacterial fitness and host-microbe
interactions. In this study, we performed a systematic
analysis of prophage elements in 2406 complete
actinobacterial genomes. Overall, 2106 prophage elements
were predicted to be present in about $50\%$ (1172/2406) of
the analyzed datasets. Interestingly, these identified
sequences compose a high prevalence of cryptic prophage
elements, indicating genetic decay and domestication.
Analysis of the sequence relationship of predicted prophages
with known actinobacteriophage genomes revealed an
exceptional high phylogenetic diversity of prophage
elements. As a trend, we observed a higher prevalence of
prophage elements in vicinity to the terminus. Analysis of
the prophage-encoded gene functions revealed that prophage
sequences significantly contribute to the bacterial
antiviral immune system, but no biosynthetic gene clusters
involved in the synthesis of known antiphage molecules were
identified in prophage genomes. Overall, the current study
highlights the remarkable diversity of prophages in
actinobacterial genomes, with highly divergent prophages in
actinobacterial genomes and thus provides an important basis
for further investigation of phage-host interactions in this
important bacterial phylum.},
cin = {IBG-1},
ddc = {600},
cid = {I:(DE-Juel1)IBG-1-20101118},
pnm = {2171 - Biological and environmental resources for
sustainable use (POF4-217)},
pid = {G:(DE-HGF)POF4-2171},
typ = {PUB:(DE-HGF)16},
pubmed = {36932119},
UT = {WOS:000955841200051},
doi = {10.1038/s41598-023-30829-z},
url = {https://juser.fz-juelich.de/record/1005578},
}