%0 Journal Article %A Kuzkina, Anastasia %A Panzer, Celine %A Seger, Aline %A Schmitt, Daniela %A Rößle, Jonas %A Schreglmann, Sebastian R. %A Knacke, Henrike %A Salabasidou, Elena %A Kohl, Antonia %A Sittig, Elisabeth %A Barbe, Michael %A Berg, Daniela %A Volkmann, Jens %A Sommer, Claudia %A Oertel, Wolfgang H. %A Schaeffer, Eva %A Sommerauer, Michael %A Janzen, Annette %A Doppler, Kathrin %T Dermal Real‐Time Quaking‐Induced Conversion Is a Sensitive Marker to Confirm Isolated Rapid Eye Movement Sleep Behavior Disorder as an Early α‐Synucleinopathy %J Movement disorders %V 38 %N 6 %@ 0885-3185 %C New York, NY %I Wiley %M FZJ-2023-01616 %P mds.29340 %D 2023 %X ABSTRACT:Background:Skin biopsy is apotential tool for the premortem confirmation of anα-synucleinopathy.Objective:The aim was to assess the aggregationassay real-time quaking-induced conversion (RT-QuIC)of skin biopsy lysates to confirm isolated rapid eyemovement sleep behavior disorder (iRBD) as anα-synucleinopathy.Methods:Skin biopsies of patients with iRBD,Parkinson’s disease (PD), and controls were analyzedusing RT-QuIC and immunohistochemical detection ofphospho-α-synuclein.Results:α-Synuclein aggregation was detected in97.4% of iRBD patients (78.4% of iRBD biopsies),87.2% of PD patients (70% of PD biopsies), and 13%of controls (7.9% of control biopsies), with a higherseeding activity in iRBD compared to PD. RT-QuICwas more sensitive but less specific thanimmunohistochemistry.Conclusions:Dermal RT-QuIC is a sensitive methodto detectα-synuclein aggregation in iRBD, and highseeding activity may indicate a strong involvement ofdermal nervefibers in these patients. © 2023 TheAuthors.Movement Disorderspublished by WileyPeriodicals LLC on behalf of International Parkinsonand Movement Disorder Society.Key Words:rapid eye movement sleep behavior dis-order;α-synuclein; Parkinson’s disease; real-timequaking-induced conversion; skin biopsy %F PUB:(DE-HGF)16 %9 Journal Article %$ 36750755 %U <Go to ISI:>//WOS:000930736600001 %R 10.1002/mds.29340 %U https://juser.fz-juelich.de/record/1005762