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001006638 1001_ $$0P:(DE-HGF)0$$aHoffmann, Marco$$b0$$eCorresponding author
001006638 245__ $$aSmuggling on the Nanoscale—Fusogenic Liposomes Enable Efficient RNA-Transfer with Negligible Immune Response In Vitro and In Vivo
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001006638 520__ $$aThe efficient and biocompatible transfer of nucleic acids into mammalian cells for research applications or medical purposes is a long-standing, challenging task. Viral transduction is the most efficient transfer system, but often entails high safety levels for research and potential health impairments for patients in medical applications. Lipo- or polyplexes are commonly used transfer systems but result in comparably low transfer efficiencies. Moreover, inflammatory responses caused by cytotoxic side effects were reported for these transfer methods. Often accountable for these effects are various recognition mechanisms for transferred nucleic acids. Using commercially available fusogenic liposomes (Fuse-It-mRNA), we established highly efficient and fully biocompatible transfer of RNA molecules for in vitro as well as in vivo applications. We demonstrated bypassing of endosomal uptake routes and, therefore, of pattern recognition receptors that recognize nucleic acids with high efficiency. This may underlie the observed almost complete abolishment of inflammatory cytokine responses. RNA transfer experiments into zebrafish embryos and adult animals fully confirmed the functional mechanism and the wide range of applications from single cells to organisms.
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001006638 7001_ $$0P:(DE-Juel1)171121$$aGerlach, Sven$$b1$$ufzj
001006638 7001_ $$00000-0002-6524-5813$$aTakamiya, Masanari$$b2
001006638 7001_ $$0P:(DE-Juel1)176880$$aTarazi, Samar$$b3
001006638 7001_ $$0P:(DE-Juel1)128815$$aHersch, Nils$$b4$$ufzj
001006638 7001_ $$0P:(DE-Juel1)128805$$aCsiszár, Agnes$$b5$$ufzj
001006638 7001_ $$0P:(DE-Juel1)144199$$aSpringer, Ronald$$b6$$ufzj
001006638 7001_ $$0P:(DE-Juel1)129308$$aDreissen, Georg$$b7$$ufzj
001006638 7001_ $$aScharr, Hanno$$b8
001006638 7001_ $$00000-0003-4411-5646$$aRastegar, Sepand$$b9
001006638 7001_ $$0P:(DE-HGF)0$$aBeil, Tanja$$b10
001006638 7001_ $$0P:(DE-HGF)0$$aSträhle, Uwe$$b11
001006638 7001_ $$0P:(DE-Juel1)128833$$aMerkel, Rudolf$$b12
001006638 7001_ $$0P:(DE-Juel1)128817$$aHoffmann, Bernd$$b13$$eCorresponding author
001006638 773__ $$0PERI:(DE-600)2527217-2$$a10.3390/pharmaceutics15041210$$gVol. 15, no. 4, p. 1210 -$$n4$$p1210 -$$tPharmaceutics$$v15$$x1999-4923$$y2023
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