Journal Article

FZJ-2023-01771

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Smuggling on the Nanoscale—Fusogenic Liposomes Enable Efficient RNA-Transfer with Negligible Immune Response In Vitro and In Vivo

Hoffmann, M. (Corresponding author)RWTH* ; Gerlach, S.FZJ* ; Takamiya, M. ; Tarazi, S.FZJ* ; Hersch, N.FZJ* ; Csiszár, A.FZJ* ; Springer, R.FZJ* ; Dreissen, G.FZJ* ; Scharr, H. ; Rastegar, S. ; Beil, T. ; Strähle, U. ; Merkel, R.FZJ* ; Hoffmann, B. (Corresponding author)FZJ*

2023
MDPI Basel

This record in other databases:      

Please use a persistent id in citations: http://hdl.handle.net/2128/34277  doi:10.3390/pharmaceutics15041210

Abstract: The efficient and biocompatible transfer of nucleic acids into mammalian cells for research applications or medical purposes is a long-standing, challenging task. Viral transduction is the most efficient transfer system, but often entails high safety levels for research and potential health impairments for patients in medical applications. Lipo- or polyplexes are commonly used transfer systems but result in comparably low transfer efficiencies. Moreover, inflammatory responses caused by cytotoxic side effects were reported for these transfer methods. Often accountable for these effects are various recognition mechanisms for transferred nucleic acids. Using commercially available fusogenic liposomes (Fuse-It-mRNA), we established highly efficient and fully biocompatible transfer of RNA molecules for in vitro as well as in vivo applications. We demonstrated bypassing of endosomal uptake routes and, therefore, of pattern recognition receptors that recognize nucleic acids with high efficiency. This may underlie the observed almost complete abolishment of inflammatory cytokine responses. RNA transfer experiments into zebrafish embryos and adult animals fully confirmed the functional mechanism and the wide range of applications from single cells to organisms.

---

Contributing Institute(s):

1. Mechanobiologie (IBI-2)
2. Datenanalyse und Maschinenlernen (IAS-8)

Research Program(s):

1. 5243 - Information Processing in Distributed Systems (POF4-524) (POF4-524)

Appears in the scientific report 2023

---

Database coverage:
; ; ; ; Article Processing Charges ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; DOAJ Seal ; Ebsco Academic Search ; Essential Science Indicators ; Fees ; IF >= 5 ; JCR ; PubMed Central ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

---