TY  - JOUR
AU  - Hoffmann, Marco
AU  - Gerlach, Sven
AU  - Takamiya, Masanari
AU  - Tarazi, Samar
AU  - Hersch, Nils
AU  - Csiszár, Agnes
AU  - Springer, Ronald
AU  - Dreissen, Georg
AU  - Scharr, Hanno
AU  - Rastegar, Sepand
AU  - Beil, Tanja
AU  - Strähle, Uwe
AU  - Merkel, Rudolf
AU  - Hoffmann, Bernd
TI  - Smuggling on the Nanoscale—Fusogenic Liposomes Enable Efficient RNA-Transfer with Negligible Immune Response In Vitro and In Vivo
JO  - Pharmaceutics
VL  - 15
IS  - 4
SN  - 1999-4923
CY  - Basel
PB  - MDPI
M1  - FZJ-2023-01771
SP  - 1210 -
PY  - 2023
AB  - The efficient and biocompatible transfer of nucleic acids into mammalian cells for research applications or medical purposes is a long-standing, challenging task. Viral transduction is the most efficient transfer system, but often entails high safety levels for research and potential health impairments for patients in medical applications. Lipo- or polyplexes are commonly used transfer systems but result in comparably low transfer efficiencies. Moreover, inflammatory responses caused by cytotoxic side effects were reported for these transfer methods. Often accountable for these effects are various recognition mechanisms for transferred nucleic acids. Using commercially available fusogenic liposomes (Fuse-It-mRNA), we established highly efficient and fully biocompatible transfer of RNA molecules for in vitro as well as in vivo applications. We demonstrated bypassing of endosomal uptake routes and, therefore, of pattern recognition receptors that recognize nucleic acids with high efficiency. This may underlie the observed almost complete abolishment of inflammatory cytokine responses. RNA transfer experiments into zebrafish embryos and adult animals fully confirmed the functional mechanism and the wide range of applications from single cells to organisms.
LB  - PUB:(DE-HGF)16
C6  - 37111695
UR  - <Go to ISI:>//WOS:000977537800001
DO  - DOI:10.3390/pharmaceutics15041210
UR  - https://juser.fz-juelich.de/record/1006638
ER  -