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@ARTICLE{Hoffmann:1006638,
      author       = {Hoffmann, Marco and Gerlach, Sven and Takamiya, Masanari
                      and Tarazi, Samar and Hersch, Nils and Csiszár, Agnes and
                      Springer, Ronald and Dreissen, Georg and Scharr, Hanno and
                      Rastegar, Sepand and Beil, Tanja and Strähle, Uwe and
                      Merkel, Rudolf and Hoffmann, Bernd},
      title        = {{S}muggling on the {N}anoscale—{F}usogenic {L}iposomes
                      {E}nable {E}fficient {RNA}-{T}ransfer with {N}egligible
                      {I}mmune {R}esponse {I}n {V}itro and {I}n {V}ivo},
      journal      = {Pharmaceutics},
      volume       = {15},
      number       = {4},
      issn         = {1999-4923},
      address      = {Basel},
      publisher    = {MDPI},
      reportid     = {FZJ-2023-01771},
      pages        = {1210 -},
      year         = {2023},
      abstract     = {The efficient and biocompatible transfer of nucleic acids
                      into mammalian cells for research applications or medical
                      purposes is a long-standing, challenging task. Viral
                      transduction is the most efficient transfer system, but
                      often entails high safety levels for research and potential
                      health impairments for patients in medical applications.
                      Lipo- or polyplexes are commonly used transfer systems but
                      result in comparably low transfer efficiencies. Moreover,
                      inflammatory responses caused by cytotoxic side effects were
                      reported for these transfer methods. Often accountable for
                      these effects are various recognition mechanisms for
                      transferred nucleic acids. Using commercially available
                      fusogenic liposomes (Fuse-It-mRNA), we established highly
                      efficient and fully biocompatible transfer of RNA molecules
                      for in vitro as well as in vivo applications. We
                      demonstrated bypassing of endosomal uptake routes and,
                      therefore, of pattern recognition receptors that recognize
                      nucleic acids with high efficiency. This may underlie the
                      observed almost complete abolishment of inflammatory
                      cytokine responses. RNA transfer experiments into zebrafish
                      embryos and adult animals fully confirmed the functional
                      mechanism and the wide range of applications from single
                      cells to organisms.},
      cin          = {IBI-2 / IAS-8},
      ddc          = {610},
      cid          = {I:(DE-Juel1)IBI-2-20200312 / I:(DE-Juel1)IAS-8-20210421},
      pnm          = {5243 - Information Processing in Distributed Systems
                      (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5243},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {37111695},
      UT           = {WOS:000977537800001},
      doi          = {10.3390/pharmaceutics15041210},
      url          = {https://juser.fz-juelich.de/record/1006638},
}