TY  - JOUR
AU  - Bitzenhofer, Nora Lisa
AU  - Höfel, Carolin
AU  - Thies, Stephan
AU  - Weiler, Andrea Jeanette
AU  - Eberlein, Christian
AU  - Heipieper, Hermann J.
AU  - Batra-Safferling, Renu
AU  - Sundermeyer, Pia
AU  - Heidler, Thomas
AU  - Sachse, Carsten
AU  - Busche, Tobias
AU  - Kalinowski, Jörn
AU  - Belthle, Thomke
AU  - Drepper, Thomas
AU  - Jaeger, Karl-Erich
AU  - Loeschcke, Anita
TI  - Exploring engineered vesiculation by Pseudomonas putida KT2440 for natural product biosynthesis
JO  - Microbial biotechnology
VL  - 17
IS  - 1
SN  - 1751-7907
CY  - Oxford
PB  - Wiley-Blackwell
M1  - FZJ-2023-02659
SP  - e14312
PY  - 2024
AB  - Pseudomonas species have become promising cell factories for the production of natural products due to their inherent robustness. Although these bacteria have naturally evolved strategies to cope with different kinds of stress, many biotechnological applications benefit from engineering of optimised chassis strains with specially adapted tolerance traits. Here, we explored the formation of outer membrane vesicles (OMV) of Pseudomonas putida KT2440. We found OMV production to correlate with the recombinant production of a natural compound with versatile beneficial properties, the tripyrrole prodigiosin. Further, several P. putida genes were identified, whose up- or down-regulated expression allowed controlling OMV formation. Finally, genetically triggering vesiculation in production strains of the different alkaloids prodigiosin, violacein, and phenazine-1-carboxylic acid, as well as the carotenoid zeaxanthin, resulted in up to three-fold increased product yields. Consequently, our findings suggest that the construction of robust strains by genetic manipulation of OMV formation might be developed into a useful tool which may contribute to improving limited biotechnological applications.
LB  - PUB:(DE-HGF)16
C6  - 37435812
UR  - <Go to ISI:>//WOS:001028928400001
DO  - DOI:10.1111/1751-7915.14312
UR  - https://juser.fz-juelich.de/record/1009129
ER  -