Journal Article

FZJ-2023-02659

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Exploring engineered vesiculation by Pseudomonas putida KT2440 for natural product biosynthesis

Bitzenhofer, N. L.FZJ* ; Höfel, C. ; Thies, S.FZJ* ; Weiler, A. J.FZJ* ; Eberlein, C. ; Heipieper, H. J. ; Batra-Safferling, R.FZJ* ; Sundermeyer, P.FZJ* ; Heidler, T.FZJ* ; Sachse, C.FZJ* ; Busche, T. ; Kalinowski, J. ; Belthle, T. ; Drepper, T.FZJ* ; Jaeger, K.-E.FZJ* ; Loeschcke, A. (Corresponding author)FZJ*

2024
Wiley-Blackwell Oxford

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Please use a persistent id in citations: doi:10.1111/1751-7915.14312  doi:10.34734/FZJ-2023-02659

Abstract: Pseudomonas species have become promising cell factories for the production of natural products due to their inherent robustness. Although these bacteria have naturally evolved strategies to cope with different kinds of stress, many biotechnological applications benefit from engineering of optimised chassis strains with specially adapted tolerance traits. Here, we explored the formation of outer membrane vesicles (OMV) of Pseudomonas putida KT2440. We found OMV production to correlate with the recombinant production of a natural compound with versatile beneficial properties, the tripyrrole prodigiosin. Further, several P. putida genes were identified, whose up- or down-regulated expression allowed controlling OMV formation. Finally, genetically triggering vesiculation in production strains of the different alkaloids prodigiosin, violacein, and phenazine-1-carboxylic acid, as well as the carotenoid zeaxanthin, resulted in up to three-fold increased product yields. Consequently, our findings suggest that the construction of robust strains by genetic manipulation of OMV formation might be developed into a useful tool which may contribute to improving limited biotechnological applications.

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Contributing Institute(s):

1. Strukturbiologie (ER-C-3)
2. Strukturbiochemie (IBI-7)
3. Institut für Molekulare Enzymtechnologie (HHUD) (IMET)

Research Program(s):

1. 5352 - Understanding the Functionality of Soft Matter and Biomolecular Systems (POF4-535) (POF4-535)
2. 5241 - Molecular Information Processing in Cellular Systems (POF4-524) (POF4-524)
3. 2171 - Biological and environmental resources for sustainable use (POF4-217) (POF4-217)

Appears in the scientific report 2024

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