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@ARTICLE{Bitzenhofer:1009129,
      author       = {Bitzenhofer, Nora Lisa and Höfel, Carolin and Thies,
                      Stephan and Weiler, Andrea Jeanette and Eberlein, Christian
                      and Heipieper, Hermann J. and Batra-Safferling, Renu and
                      Sundermeyer, Pia and Heidler, Thomas and Sachse, Carsten and
                      Busche, Tobias and Kalinowski, Jörn and Belthle, Thomke and
                      Drepper, Thomas and Jaeger, Karl-Erich and Loeschcke, Anita},
      title        = {{E}xploring engineered vesiculation by {P}seudomonas putida
                      {KT}2440 for natural product biosynthesis},
      journal      = {Microbial biotechnology},
      volume       = {17},
      number       = {1},
      issn         = {1751-7907},
      address      = {Oxford},
      publisher    = {Wiley-Blackwell},
      reportid     = {FZJ-2023-02659},
      pages        = {e14312},
      year         = {2024},
      abstract     = {Pseudomonas species have become promising cell factories
                      for the production of natural products due to their inherent
                      robustness. Although these bacteria have naturally evolved
                      strategies to cope with different kinds of stress, many
                      biotechnological applications benefit from engineering of
                      optimised chassis strains with specially adapted tolerance
                      traits. Here, we explored the formation of outer membrane
                      vesicles (OMV) of Pseudomonas putida KT2440. We found OMV
                      production to correlate with the recombinant production of a
                      natural compound with versatile beneficial properties, the
                      tripyrrole prodigiosin. Further, several P. putida genes
                      were identified, whose up- or down-regulated expression
                      allowed controlling OMV formation. Finally, genetically
                      triggering vesiculation in production strains of the
                      different alkaloids prodigiosin, violacein, and
                      phenazine-1-carboxylic acid, as well as the carotenoid
                      zeaxanthin, resulted in up to three-fold increased product
                      yields. Consequently, our findings suggest that the
                      construction of robust strains by genetic manipulation of
                      OMV formation might be developed into a useful tool which
                      may contribute to improving limited biotechnological
                      applications.},
      cin          = {ER-C-3 / IBI-7 / IMET},
      ddc          = {610},
      cid          = {I:(DE-Juel1)ER-C-3-20170113 / I:(DE-Juel1)IBI-7-20200312 /
                      I:(DE-Juel1)IMET-20090612},
      pnm          = {5352 - Understanding the Functionality of Soft Matter and
                      Biomolecular Systems (POF4-535) / 5241 - Molecular
                      Information Processing in Cellular Systems (POF4-524) / 2171
                      - Biological and environmental resources for sustainable use
                      (POF4-217)},
      pid          = {G:(DE-HGF)POF4-5352 / G:(DE-HGF)POF4-5241 /
                      G:(DE-HGF)POF4-2171},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {37435812},
      UT           = {WOS:001028928400001},
      doi          = {10.1111/1751-7915.14312},
      url          = {https://juser.fz-juelich.de/record/1009129},
}