%0 Journal Article
%A Glueck, David
%A Grethen, Anne
%A Das, Manabendra
%A Mmeka, Ogochukwu Patricia
%A Patallo, Eugenio Pérez
%A Meister, Annette
%A Rajender, Ritu
%A Kins, Stefan
%A Räschle, Markus
%A Victor, Julian
%A Chu, Ci
%A Etzkorn, Manuel
%A Köck, Zoe
%A Bernhard, Frank
%A Babalola, Jonathan Oyebamiji
%A Vargas, Carolyn
%A Keller, Sandro
%T Electroneutral Polymer Nanodiscs Enable Interference‐Free Probing of Membrane Proteins in a Lipid‐Bilayer Environment
%J Small
%V 18
%N 47
%@ 1613-6810
%C Weinheim
%I Wiley-VCH
%M FZJ-2023-03929
%P 2202492
%D 2022
%X Membrane proteins can be examined in near-native lipid-bilayer environments with the advent of polymer-encapsulated nanodiscs. These nanodiscs self-assemble directly from cellular membranes, allowing in vitro probing of membrane proteins with techniques that have previously been restricted to soluble or detergent-solubilized proteins. Often, however, the high charge densities of existing polymers obstruct bioanalytical and preparative techniques. Thus, the authors aim to fabricate electroneutral—yet water-soluble—polymer nanodiscs. By attaching a sulfobetaine group to the commercial polymers DIBMA and SMA(2:1), these polyanionic polymers are converted to the electroneutral maleimide derivatives, Sulfo-DIBMA and Sulfo-SMA(2:1). Sulfo-DIBMA and Sulfo-SMA(2:1) readily extract proteins and phospholipids from artificial and cellular membranes to form nanodiscs. Crucially, the electroneutral nanodiscs avert unspecific interactions, thereby enabling new insights into protein–lipid interactions through lab-on-a-chip detection and in vitro translation of membrane proteins. Finally, the authors create a library comprising thousands of human membrane proteins and use proteome profiling by mass spectrometry to show that protein complexes are preserved in electroneutral nanodiscs.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ 36228092
%U <Go to ISI:>//WOS:000867936900001
%R 10.1002/smll.202202492
%U https://juser.fz-juelich.de/record/1017087