Journal Article

FZJ-2023-03929

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Electroneutral Polymer Nanodiscs Enable Interference‐Free Probing of Membrane Proteins in a Lipid‐Bilayer Environment

Glueck, D. ; Grethen, A. ; Das, M. ; Mmeka, O. P. ; Patallo, E. P. ; Meister, A. ; Rajender, R. ; Kins, S. ; Räschle, M. ; Victor, J. ; Chu, C. ; Etzkorn, M.FZJ* ; Köck, Z. ; Bernhard, F. ; Babalola, J. O. ; Vargas, C. ; Keller, S. (Corresponding author)

2022
Wiley-VCH Weinheim

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Please use a persistent id in citations: doi:10.1002/smll.202202492  doi:10.34734/FZJ-2023-03929

Abstract: Membrane proteins can be examined in near-native lipid-bilayer environments with the advent of polymer-encapsulated nanodiscs. These nanodiscs self-assemble directly from cellular membranes, allowing in vitro probing of membrane proteins with techniques that have previously been restricted to soluble or detergent-solubilized proteins. Often, however, the high charge densities of existing polymers obstruct bioanalytical and preparative techniques. Thus, the authors aim to fabricate electroneutral—yet water-soluble—polymer nanodiscs. By attaching a sulfobetaine group to the commercial polymers DIBMA and SMA(2:1), these polyanionic polymers are converted to the electroneutral maleimide derivatives, Sulfo-DIBMA and Sulfo-SMA(2:1). Sulfo-DIBMA and Sulfo-SMA(2:1) readily extract proteins and phospholipids from artificial and cellular membranes to form nanodiscs. Crucially, the electroneutral nanodiscs avert unspecific interactions, thereby enabling new insights into protein–lipid interactions through lab-on-a-chip detection and in vitro translation of membrane proteins. Finally, the authors create a library comprising thousands of human membrane proteins and use proteome profiling by mass spectrometry to show that protein complexes are preserved in electroneutral nanodiscs.

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Contributing Institute(s):

1. Strukturbiochemie (IBI-7)

Research Program(s):

1. 5241 - Molecular Information Processing in Cellular Systems (POF4-524) (POF4-524)

Appears in the scientific report 2023

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Database coverage:
; ; ; Clarivate Analytics Master Journal List ; Current Contents - Physical, Chemical and Earth Sciences ; DEAL Wiley ; Essential Science Indicators ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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