TY  - JOUR
AU  - Glueck, David
AU  - Grethen, Anne
AU  - Das, Manabendra
AU  - Mmeka, Ogochukwu Patricia
AU  - Patallo, Eugenio Pérez
AU  - Meister, Annette
AU  - Rajender, Ritu
AU  - Kins, Stefan
AU  - Räschle, Markus
AU  - Victor, Julian
AU  - Chu, Ci
AU  - Etzkorn, Manuel
AU  - Köck, Zoe
AU  - Bernhard, Frank
AU  - Babalola, Jonathan Oyebamiji
AU  - Vargas, Carolyn
AU  - Keller, Sandro
TI  - Electroneutral Polymer Nanodiscs Enable Interference‐Free Probing of Membrane Proteins in a Lipid‐Bilayer Environment
JO  - Small
VL  - 18
IS  - 47
SN  - 1613-6810
CY  - Weinheim
PB  - Wiley-VCH
M1  - FZJ-2023-03929
SP  - 2202492
PY  - 2022
AB  - Membrane proteins can be examined in near-native lipid-bilayer environments with the advent of polymer-encapsulated nanodiscs. These nanodiscs self-assemble directly from cellular membranes, allowing in vitro probing of membrane proteins with techniques that have previously been restricted to soluble or detergent-solubilized proteins. Often, however, the high charge densities of existing polymers obstruct bioanalytical and preparative techniques. Thus, the authors aim to fabricate electroneutral—yet water-soluble—polymer nanodiscs. By attaching a sulfobetaine group to the commercial polymers DIBMA and SMA(2:1), these polyanionic polymers are converted to the electroneutral maleimide derivatives, Sulfo-DIBMA and Sulfo-SMA(2:1). Sulfo-DIBMA and Sulfo-SMA(2:1) readily extract proteins and phospholipids from artificial and cellular membranes to form nanodiscs. Crucially, the electroneutral nanodiscs avert unspecific interactions, thereby enabling new insights into protein–lipid interactions through lab-on-a-chip detection and in vitro translation of membrane proteins. Finally, the authors create a library comprising thousands of human membrane proteins and use proteome profiling by mass spectrometry to show that protein complexes are preserved in electroneutral nanodiscs.
LB  - PUB:(DE-HGF)16
C6  - 36228092
UR  - <Go to ISI:>//WOS:000867936900001
DO  - DOI:10.1002/smll.202202492
UR  - https://juser.fz-juelich.de/record/1017087
ER  -