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@ARTICLE{Glueck:1017087,
author = {Glueck, David and Grethen, Anne and Das, Manabendra and
Mmeka, Ogochukwu Patricia and Patallo, Eugenio Pérez and
Meister, Annette and Rajender, Ritu and Kins, Stefan and
Räschle, Markus and Victor, Julian and Chu, Ci and Etzkorn,
Manuel and Köck, Zoe and Bernhard, Frank and Babalola,
Jonathan Oyebamiji and Vargas, Carolyn and Keller, Sandro},
title = {{E}lectroneutral {P}olymer {N}anodiscs {E}nable
{I}nterference‐{F}ree {P}robing of {M}embrane {P}roteins
in a {L}ipid‐{B}ilayer {E}nvironment},
journal = {Small},
volume = {18},
number = {47},
issn = {1613-6810},
address = {Weinheim},
publisher = {Wiley-VCH},
reportid = {FZJ-2023-03929},
pages = {2202492},
year = {2022},
abstract = {Membrane proteins can be examined in near-native
lipid-bilayer environments with the advent of
polymer-encapsulated nanodiscs. These nanodiscs
self-assemble directly from cellular membranes, allowing in
vitro probing of membrane proteins with techniques that have
previously been restricted to soluble or
detergent-solubilized proteins. Often, however, the high
charge densities of existing polymers obstruct bioanalytical
and preparative techniques. Thus, the authors aim to
fabricate electroneutral—yet water-soluble—polymer
nanodiscs. By attaching a sulfobetaine group to the
commercial polymers DIBMA and SMA(2:1), these polyanionic
polymers are converted to the electroneutral maleimide
derivatives, Sulfo-DIBMA and Sulfo-SMA(2:1). Sulfo-DIBMA and
Sulfo-SMA(2:1) readily extract proteins and phospholipids
from artificial and cellular membranes to form nanodiscs.
Crucially, the electroneutral nanodiscs avert unspecific
interactions, thereby enabling new insights into
protein–lipid interactions through lab-on-a-chip detection
and in vitro translation of membrane proteins. Finally, the
authors create a library comprising thousands of human
membrane proteins and use proteome profiling by mass
spectrometry to show that protein complexes are preserved in
electroneutral nanodiscs.},
cin = {IBI-7},
ddc = {620},
cid = {I:(DE-Juel1)IBI-7-20200312},
pnm = {5241 - Molecular Information Processing in Cellular Systems
(POF4-524)},
pid = {G:(DE-HGF)POF4-5241},
typ = {PUB:(DE-HGF)16},
pubmed = {36228092},
UT = {WOS:000867936900001},
doi = {10.1002/smll.202202492},
url = {https://juser.fz-juelich.de/record/1017087},
}