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| 001 | 1017087 | ||
| 005 | 20240226075510.0 | ||
| 024 | 7 | _ | |a 10.1002/smll.202202492 |2 doi |
| 024 | 7 | _ | |a 1613-6810 |2 ISSN |
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| 100 | 1 | _ | |a Glueck, David |0 0000-0003-3983-1277 |b 0 |
| 245 | _ | _ | |a Electroneutral Polymer Nanodiscs Enable Interference‐Free Probing of Membrane Proteins in a Lipid‐Bilayer Environment |
| 260 | _ | _ | |a Weinheim |c 2022 |b Wiley-VCH |
| 336 | 7 | _ | |a article |2 DRIVER |
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| 520 | _ | _ | |a Membrane proteins can be examined in near-native lipid-bilayer environments with the advent of polymer-encapsulated nanodiscs. These nanodiscs self-assemble directly from cellular membranes, allowing in vitro probing of membrane proteins with techniques that have previously been restricted to soluble or detergent-solubilized proteins. Often, however, the high charge densities of existing polymers obstruct bioanalytical and preparative techniques. Thus, the authors aim to fabricate electroneutral—yet water-soluble—polymer nanodiscs. By attaching a sulfobetaine group to the commercial polymers DIBMA and SMA(2:1), these polyanionic polymers are converted to the electroneutral maleimide derivatives, Sulfo-DIBMA and Sulfo-SMA(2:1). Sulfo-DIBMA and Sulfo-SMA(2:1) readily extract proteins and phospholipids from artificial and cellular membranes to form nanodiscs. Crucially, the electroneutral nanodiscs avert unspecific interactions, thereby enabling new insights into protein–lipid interactions through lab-on-a-chip detection and in vitro translation of membrane proteins. Finally, the authors create a library comprising thousands of human membrane proteins and use proteome profiling by mass spectrometry to show that protein complexes are preserved in electroneutral nanodiscs. |
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| 700 | 1 | _ | |a Grethen, Anne |0 P:(DE-HGF)0 |b 1 |
| 700 | 1 | _ | |a Das, Manabendra |0 0000-0003-3690-3183 |b 2 |
| 700 | 1 | _ | |a Mmeka, Ogochukwu Patricia |0 P:(DE-HGF)0 |b 3 |
| 700 | 1 | _ | |a Patallo, Eugenio Pérez |0 P:(DE-HGF)0 |b 4 |
| 700 | 1 | _ | |a Meister, Annette |0 0000-0003-1668-4382 |b 5 |
| 700 | 1 | _ | |a Rajender, Ritu |0 P:(DE-HGF)0 |b 6 |
| 700 | 1 | _ | |a Kins, Stefan |0 P:(DE-HGF)0 |b 7 |
| 700 | 1 | _ | |a Räschle, Markus |0 0000-0002-6737-7203 |b 8 |
| 700 | 1 | _ | |a Victor, Julian |b 9 |
| 700 | 1 | _ | |a Chu, Ci |0 P:(DE-HGF)0 |b 10 |
| 700 | 1 | _ | |a Etzkorn, Manuel |0 P:(DE-Juel1)156341 |b 11 |
| 700 | 1 | _ | |a Köck, Zoe |0 P:(DE-HGF)0 |b 12 |
| 700 | 1 | _ | |a Bernhard, Frank |0 0000-0002-4860-1783 |b 13 |
| 700 | 1 | _ | |a Babalola, Jonathan Oyebamiji |0 0000-0002-1407-6677 |b 14 |
| 700 | 1 | _ | |a Vargas, Carolyn |0 P:(DE-HGF)0 |b 15 |
| 700 | 1 | _ | |a Keller, Sandro |0 0000-0001-5469-8772 |b 16 |e Corresponding author |
| 773 | _ | _ | |a 10.1002/smll.202202492 |g Vol. 18, no. 47, p. 2202492 |0 PERI:(DE-600)2168935-0 |n 47 |p 2202492 |t Small |v 18 |y 2022 |x 1613-6810 |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/1017087/files/Small%20-%202022%20-%20Glueck%20-%20Electroneutral%20Polymer%20Nanodiscs%20Enable%20Interference%E2%80%90Free%20Probing%20of%20Membrane%20Proteins%20in%20a.pdf |y OpenAccess |
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