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| Home > Publications database > 11C-labelling in combination with Positron Emission Tomography (PET) for investigating carbon transport dynamics |
| Conference Presentation (After Call) | FZJ-2023-05066 |
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2023
Abstract: 11C-labelling in combination with Positron Emission Tomography (PET) can be used for investigating carbon transport dynamics even in complex 3D plant organs above and belowground The short-lived radioisotope 11C can be applied non-invasively to the plant as 11CO2 to monitor the transport of recently fixed carbon and its allocation within the plant. The combination of the 11C tracer with PET detection, compartmental modelling, and Magnetic Resonance Imaging (MRI) enables the imaging and quantification of carbon transport velocities and allocation in complex 3D structures, such as the root system or branched shoots and fruits. A controlled administration of tracer and growth conditions of plants, provide repeatability to address mechanistic questions of carbon flow and allocation. We will present results on carbon transport velocities in stems and roots of various plants. For bean plants that were measured 3 times per day over a period of 4 days we found that tracer transport velocities ranged between 3 and 8 mm*min-1 and were strongly dependent on growth stage of the plant and source leaf. Also, different consecutive organs (e.g., petiole, branch, stem, or root) along the transport pathway from a single labelled leaf showed varying transport velocities. For comparing different positions, PET is advantageous as several plant organs can be imaged in parallel without consecutive measurements. Similar datasets for poplar which showed overall faster transport velocities will be compared with the bean data. We will further present parallel measurements of roots on the example of maize, as a particularly complex system exhibiting strong root-type differences and sugar beet as a large storage organ with parallel transport pathways. Here MRI was widely used to characterize the root structure belowground, before, during and after the PET measurements, allowing for added information on the 3D structure belowground. First data on quantification of a treatment effect (leaf shading) on carbon transport velocity will be presented briefly.Our results highlight the advantages of 11C-labelling in combination with PET and MRI for measuring non-invasively dynamics in carbon transport velocities s in different plant organs.
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