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@INPROCEEDINGS{Metzner:1018997,
      author       = {Metzner, Ralf and Chlubek, Antonia and Pflugfelder, Daniel
                      and Schurr, Ulrich and Huber, Gregor and Windt, Carel and
                      Koller, Robert},
      title        = {11{C}-labelling in combination with {P}ositron {E}mission
                      {T}omography ({PET}) for investigating carbon transport
                      dynamics},
      reportid     = {FZJ-2023-05066},
      year         = {2023},
      abstract     = {11C-labelling in combination with Positron Emission
                      Tomography (PET) can be used for investigating carbon
                      transport dynamics even in complex 3D plant organs above and
                      belowground The short-lived radioisotope 11C can be applied
                      non-invasively to the plant as 11CO2 to monitor the
                      transport of recently fixed carbon and its allocation within
                      the plant. The combination of the 11C tracer with PET
                      detection, compartmental modelling, and Magnetic Resonance
                      Imaging (MRI) enables the imaging and quantification of
                      carbon transport velocities and allocation in complex 3D
                      structures, such as the root system or branched shoots and
                      fruits. A controlled administration of tracer and growth
                      conditions of plants, provide repeatability to address
                      mechanistic questions of carbon flow and allocation. We will
                      present results on carbon transport velocities in stems and
                      roots of various plants. For bean plants that were measured
                      3 times per day over a period of 4 days we found that tracer
                      transport velocities ranged between 3 and 8 mm*min-1 and
                      were strongly dependent on growth stage of the plant and
                      source leaf. Also, different consecutive organs (e.g.,
                      petiole, branch, stem, or root) along the transport pathway
                      from a single labelled leaf showed varying transport
                      velocities. For comparing different positions, PET is
                      advantageous as several plant organs can be imaged in
                      parallel without consecutive measurements. Similar datasets
                      for poplar which showed overall faster transport velocities
                      will be compared with the bean data. We will further present
                      parallel measurements of roots on the example of maize, as a
                      particularly complex system exhibiting strong root-type
                      differences and sugar beet as a large storage organ with
                      parallel transport pathways. Here MRI was widely used to
                      characterize the root structure belowground, before, during
                      and after the PET measurements, allowing for added
                      information on the 3D structure belowground. First data on
                      quantification of a treatment effect (leaf shading) on
                      carbon transport velocity will be presented briefly.Our
                      results highlight the advantages of 11C-labelling in
                      combination with PET and MRI for measuring non-invasively
                      dynamics in carbon transport velocities s in different plant
                      organs.},
      month         = {Nov},
      date          = {2023-11-20},
      organization  = {2nd Workshop Carbon Allocation in
                       Plants - Advances in carbon allocation
                       and acquisition, Versailles (France),
                       20 Nov 2023 - 21 Nov 2023},
      subtyp        = {After Call},
      cin          = {IBG-2},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {2171 - Biological and environmental resources for
                      sustainable use (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2171},
      typ          = {PUB:(DE-HGF)6},
      url          = {https://juser.fz-juelich.de/record/1018997},
}