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001024123 1001_ $$0P:(DE-HGF)0$$aHenderikx, Rene J. M.$$b0
001024123 245__ $$aVitroJet: new features and case studies
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001024123 520__ $$aSingle-particle cryo-electron microscopy has become a widely adopted methodin structural biology due to many recent technological advances in microscopes,detectors and image processing. Before being able to inspect a biological samplein an electron microscope, it needs to be deposited in a thin layer on a grid andrapidly frozen. The VitroJet was designed with this aim, as well as avoiding thedelicate manual handling and transfer steps that occur during the conventionalgrid-preparation process. Since its creation, numerous technical developmentshave resulted in a device that is now widely utilized in multiple laboratoriesworldwide. It features plasma treatment, low-volume sample deposition throughpin printing, optical ice-thickness measurement and cryofixation of pre-clippedAutogrids through jet vitrification. This paper presents recent technicalimprovements to the VitroJet and the benefits that it brings to the cryo-EMworkflow. A wide variety of applications are shown: membrane proteins,nucleosomes, fatty-acid synthase, Tobacco mosaic virus, lipid nanoparticles, tickborneencephalitis viruses and bacteriophages. These case studies illustrate theadvancement of the VitroJet into an instrument that enables accurate controland reproducibility, demonstrating its suitability for time-efficient cryo-EMstructure determination.
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001024123 7001_ $$0P:(DE-Juel1)179550$$aMann, Daniel$$b1
001024123 7001_ $$0P:(DE-HGF)0$$aDomanska, Aušra$$b2
001024123 7001_ $$0P:(DE-HGF)0$$aDong, Jing$$b3
001024123 7001_ $$0P:(DE-Juel1)194807$$aShahzad, Saba$$b4$$ufzj
001024123 7001_ $$0P:(DE-HGF)0$$aLak, Behnam$$b5
001024123 7001_ $$0P:(DE-Juel1)184560$$aFilopoulou, Aikaterini$$b6$$ufzj
001024123 7001_ $$0P:(DE-HGF)0$$aLudig, Damian$$b7
001024123 7001_ $$0P:(DE-HGF)0$$aGrininger, Martin$$b8
001024123 7001_ $$0P:(DE-HGF)0$$aMomoh, Jeffrey$$b9
001024123 7001_ $$aLaanto, Elina$$b10
001024123 7001_ $$0P:(DE-HGF)0$$aOksanen, Hanna M.$$b11
001024123 7001_ $$0P:(DE-HGF)0$$aBisikalo, Kyrylo$$b12
001024123 7001_ $$0P:(DE-HGF)0$$aWilliams, Pamela A.$$b13
001024123 7001_ $$0P:(DE-HGF)0$$aButcher, Sarah J.$$b14
001024123 7001_ $$0P:(DE-Juel1)131901$$aPeters, Peter J.$$b15
001024123 7001_ $$0P:(DE-HGF)0$$aBeulen, Bart W. A. M. M.$$b16$$eCorresponding author
001024123 773__ $$0PERI:(DE-600)2968623-4$$a10.1107/S2059798324001852$$gVol. 80, no. 4$$n4$$pD80$$tActa crystallographica / Section D$$v80$$x0907-4449$$y2024
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