Journal Article

FZJ-2024-01972

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png VitroJet: new features and case studies

Henderikx, R. J. M. ; Mann, D.FZJ* ; Domanska, A. ; Dong, J. ; Shahzad, S.FZJ* ; Lak, B. ; Filopoulou, A.FZJ* ; Ludig, D. ; Grininger, M. ; Momoh, J. ; Laanto, E. ; Oksanen, H. M. ; Bisikalo, K. ; Williams, P. A. ; Butcher, S. J. ; Peters, P. J. ; Beulen, B. W. A. M. M. (Corresponding author)

2024
Wiley Bognor Regis

This record in other databases:      

Please use a persistent id in citations: doi:10.1107/S2059798324001852  doi:10.34734/FZJ-2024-01972

Abstract: Single-particle cryo-electron microscopy has become a widely adopted methodin structural biology due to many recent technological advances in microscopes,detectors and image processing. Before being able to inspect a biological samplein an electron microscope, it needs to be deposited in a thin layer on a grid andrapidly frozen. The VitroJet was designed with this aim, as well as avoiding thedelicate manual handling and transfer steps that occur during the conventionalgrid-preparation process. Since its creation, numerous technical developmentshave resulted in a device that is now widely utilized in multiple laboratoriesworldwide. It features plasma treatment, low-volume sample deposition throughpin printing, optical ice-thickness measurement and cryofixation of pre-clippedAutogrids through jet vitrification. This paper presents recent technicalimprovements to the VitroJet and the benefits that it brings to the cryo-EMworkflow. A wide variety of applications are shown: membrane proteins,nucleosomes, fatty-acid synthase, Tobacco mosaic virus, lipid nanoparticles, tickborneencephalitis viruses and bacteriophages. These case studies illustrate theadvancement of the VitroJet into an instrument that enables accurate controland reproducibility, demonstrating its suitability for time-efficient cryo-EMstructure determination.

---

Contributing Institute(s):

1. Strukturbiologie (ER-C-3)

Research Program(s):

1. 5352 - Understanding the Functionality of Soft Matter and Biomolecular Systems (POF4-535) (POF4-535)
2. 5241 - Molecular Information Processing in Cellular Systems (POF4-524) (POF4-524)

Appears in the scientific report 2024

---

Database coverage:
; ; ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; DEAL Wiley ; Ebsco Academic Search ; Essential Science Indicators ; IF < 5 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

---