TY  - JOUR
AU  - Henderikx, Rene J. M.
AU  - Mann, Daniel
AU  - Domanska, Aušra
AU  - Dong, Jing
AU  - Shahzad, Saba
AU  - Lak, Behnam
AU  - Filopoulou, Aikaterini
AU  - Ludig, Damian
AU  - Grininger, Martin
AU  - Momoh, Jeffrey
AU  - Laanto, Elina
AU  - Oksanen, Hanna M.
AU  - Bisikalo, Kyrylo
AU  - Williams, Pamela A.
AU  - Butcher, Sarah J.
AU  - Peters, Peter J.
AU  - Beulen, Bart W. A. M. M.
TI  - VitroJet: new features and case studies
JO  - Acta crystallographica / Section D
VL  - 80
IS  - 4
SN  - 0907-4449
CY  - Bognor Regis
PB  - Wiley
M1  - FZJ-2024-01972
SP  - D80
PY  - 2024
AB  - Single-particle cryo-electron microscopy has become a widely adopted methodin structural biology due to many recent technological advances in microscopes,detectors and image processing. Before being able to inspect a biological samplein an electron microscope, it needs to be deposited in a thin layer on a grid andrapidly frozen. The VitroJet was designed with this aim, as well as avoiding thedelicate manual handling and transfer steps that occur during the conventionalgrid-preparation process. Since its creation, numerous technical developmentshave resulted in a device that is now widely utilized in multiple laboratoriesworldwide. It features plasma treatment, low-volume sample deposition throughpin printing, optical ice-thickness measurement and cryofixation of pre-clippedAutogrids through jet vitrification. This paper presents recent technicalimprovements to the VitroJet and the benefits that it brings to the cryo-EMworkflow. A wide variety of applications are shown: membrane proteins,nucleosomes, fatty-acid synthase, Tobacco mosaic virus, lipid nanoparticles, tickborneencephalitis viruses and bacteriophages. These case studies illustrate theadvancement of the VitroJet into an instrument that enables accurate controland reproducibility, demonstrating its suitability for time-efficient cryo-EMstructure determination.
LB  - PUB:(DE-HGF)16
C6  - 38488730
UR  - <Go to ISI:>//WOS:001208750500002
DO  - DOI:10.1107/S2059798324001852
UR  - https://juser.fz-juelich.de/record/1024123
ER  -