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@ARTICLE{Henderikx:1024123,
      author       = {Henderikx, Rene J. M. and Mann, Daniel and Domanska, Aušra
                      and Dong, Jing and Shahzad, Saba and Lak, Behnam and
                      Filopoulou, Aikaterini and Ludig, Damian and Grininger,
                      Martin and Momoh, Jeffrey and Laanto, Elina and Oksanen,
                      Hanna M. and Bisikalo, Kyrylo and Williams, Pamela A. and
                      Butcher, Sarah J. and Peters, Peter J. and Beulen, Bart W.
                      A. M. M.},
      title        = {{V}itro{J}et: new features and case studies},
      journal      = {Acta crystallographica / Section D},
      volume       = {80},
      number       = {4},
      issn         = {0907-4449},
      address      = {Bognor Regis},
      publisher    = {Wiley},
      reportid     = {FZJ-2024-01972},
      pages        = {D80},
      year         = {2024},
      abstract     = {Single-particle cryo-electron microscopy has become a
                      widely adopted methodin structural biology due to many
                      recent technological advances in microscopes,detectors and
                      image processing. Before being able to inspect a biological
                      samplein an electron microscope, it needs to be deposited in
                      a thin layer on a grid andrapidly frozen. The VitroJet was
                      designed with this aim, as well as avoiding thedelicate
                      manual handling and transfer steps that occur during the
                      conventionalgrid-preparation process. Since its creation,
                      numerous technical developmentshave resulted in a device
                      that is now widely utilized in multiple
                      laboratoriesworldwide. It features plasma treatment,
                      low-volume sample deposition throughpin printing, optical
                      ice-thickness measurement and cryofixation of
                      pre-clippedAutogrids through jet vitrification. This paper
                      presents recent technicalimprovements to the VitroJet and
                      the benefits that it brings to the cryo-EMworkflow. A wide
                      variety of applications are shown: membrane
                      proteins,nucleosomes, fatty-acid synthase, Tobacco mosaic
                      virus, lipid nanoparticles, tickborneencephalitis viruses
                      and bacteriophages. These case studies illustrate
                      theadvancement of the VitroJet into an instrument that
                      enables accurate controland reproducibility, demonstrating
                      its suitability for time-efficient cryo-EMstructure
                      determination.},
      cin          = {ER-C-3},
      ddc          = {530},
      cid          = {I:(DE-Juel1)ER-C-3-20170113},
      pnm          = {5352 - Understanding the Functionality of Soft Matter and
                      Biomolecular Systems (POF4-535) / 5241 - Molecular
                      Information Processing in Cellular Systems (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5352 / G:(DE-HGF)POF4-5241},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {38488730},
      UT           = {WOS:001208750500002},
      doi          = {10.1107/S2059798324001852},
      url          = {https://juser.fz-juelich.de/record/1024123},
}