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| 001 | 1024123 | ||
| 005 | 20250204113815.0 | ||
| 024 | 7 | _ | |a 10.1107/S2059798324001852 |2 doi |
| 024 | 7 | _ | |a 0907-4449 |2 ISSN |
| 024 | 7 | _ | |a 1399-0047 |2 ISSN |
| 024 | 7 | _ | |a 2059-7983 |2 ISSN |
| 024 | 7 | _ | |a 10.34734/FZJ-2024-01972 |2 datacite_doi |
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| 100 | 1 | _ | |a Henderikx, Rene J. M. |0 P:(DE-HGF)0 |b 0 |
| 245 | _ | _ | |a VitroJet: new features and case studies |
| 260 | _ | _ | |a Bognor Regis |c 2024 |b Wiley |
| 336 | 7 | _ | |a article |2 DRIVER |
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| 520 | _ | _ | |a Single-particle cryo-electron microscopy has become a widely adopted methodin structural biology due to many recent technological advances in microscopes,detectors and image processing. Before being able to inspect a biological samplein an electron microscope, it needs to be deposited in a thin layer on a grid andrapidly frozen. The VitroJet was designed with this aim, as well as avoiding thedelicate manual handling and transfer steps that occur during the conventionalgrid-preparation process. Since its creation, numerous technical developmentshave resulted in a device that is now widely utilized in multiple laboratoriesworldwide. It features plasma treatment, low-volume sample deposition throughpin printing, optical ice-thickness measurement and cryofixation of pre-clippedAutogrids through jet vitrification. This paper presents recent technicalimprovements to the VitroJet and the benefits that it brings to the cryo-EMworkflow. A wide variety of applications are shown: membrane proteins,nucleosomes, fatty-acid synthase, Tobacco mosaic virus, lipid nanoparticles, tickborneencephalitis viruses and bacteriophages. These case studies illustrate theadvancement of the VitroJet into an instrument that enables accurate controland reproducibility, demonstrating its suitability for time-efficient cryo-EMstructure determination. |
| 536 | _ | _ | |a 5352 - Understanding the Functionality of Soft Matter and Biomolecular Systems (POF4-535) |0 G:(DE-HGF)POF4-5352 |c POF4-535 |f POF IV |x 0 |
| 536 | _ | _ | |a 5241 - Molecular Information Processing in Cellular Systems (POF4-524) |0 G:(DE-HGF)POF4-5241 |c POF4-524 |f POF IV |x 1 |
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| 700 | 1 | _ | |a Mann, Daniel |0 P:(DE-Juel1)179550 |b 1 |
| 700 | 1 | _ | |a Domanska, Aušra |0 P:(DE-HGF)0 |b 2 |
| 700 | 1 | _ | |a Dong, Jing |0 P:(DE-HGF)0 |b 3 |
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| 700 | 1 | _ | |a Grininger, Martin |0 P:(DE-HGF)0 |b 8 |
| 700 | 1 | _ | |a Momoh, Jeffrey |0 P:(DE-HGF)0 |b 9 |
| 700 | 1 | _ | |a Laanto, Elina |b 10 |
| 700 | 1 | _ | |a Oksanen, Hanna M. |0 P:(DE-HGF)0 |b 11 |
| 700 | 1 | _ | |a Bisikalo, Kyrylo |0 P:(DE-HGF)0 |b 12 |
| 700 | 1 | _ | |a Williams, Pamela A. |0 P:(DE-HGF)0 |b 13 |
| 700 | 1 | _ | |a Butcher, Sarah J. |0 P:(DE-HGF)0 |b 14 |
| 700 | 1 | _ | |a Peters, Peter J. |0 P:(DE-Juel1)131901 |b 15 |
| 700 | 1 | _ | |a Beulen, Bart W. A. M. M. |0 P:(DE-HGF)0 |b 16 |e Corresponding author |
| 773 | _ | _ | |a 10.1107/S2059798324001852 |g Vol. 80, no. 4 |0 PERI:(DE-600)2968623-4 |n 4 |p D80 |t Acta crystallographica / Section D |v 80 |y 2024 |x 0907-4449 |
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