TY  - JOUR
AU  - Eria-Oliveira, Ana-Sofia
AU  - Folacci, Mathilde
AU  - Chassot, Anne Amandine
AU  - Fedou, Sandrine
AU  - Thézé, Nadine
AU  - Zabelskii, Dmitrii
AU  - Alekseev, Alexey
AU  - Bamberg, Ernst
AU  - Gordeliy, Valentin
AU  - Sandoz, Guillaume
AU  - Vivaudou, Michel
TI  - Hijacking of internal calcium dynamics by intracellularly residing viral rhodopsins
JO  - Nature Communications
VL  - 15
IS  - 1
SN  - 2041-1723
CY  - [London]
PB  - Nature Publishing Group UK
M1  - FZJ-2024-03244
SP  - 65
PY  - 2024
AB  - Rhodopsins are ubiquitous light-driven membrane proteins with diverse functions, including ion transport. Widely distributed, they are also coded in the genomes of giant viruses infecting phytoplankton where their function is not settled. Here, we examine the properties of OLPVR1 (Organic Lake Phycodnavirus Rhodopsin) and two other type 1 viral channelrhodopsins (VCR1s), and demonstrate that VCR1s accumulate exclusively intracellularly, and, upon illumination, induce calcium release from intracellular IP3-dependent stores. In vivo, this light-induced calcium release is sufficient to remote control muscle contraction in VCR1-expressing tadpoles. VCR1s natively confer light-induced Ca2+ release, suggesting a distinct mechanism for reshaping the response to light of virus-infected algae. The ability of VCR1s to photorelease calcium without altering plasma membrane electrical properties marks them as potential precursors for optogenetics tools, with potential applications in basic research and medicine.
LB  - PUB:(DE-HGF)16
C6  - 38167346
UR  - <Go to ISI:>//WOS:001288614600035
DO  - DOI:10.1038/s41467-023-44548-6
UR  - https://juser.fz-juelich.de/record/1025966
ER  -