Journal Article

FZJ-2024-03244

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Hijacking of internal calcium dynamics by intracellularly residing viral rhodopsins

Eria-Oliveira, A.-S. ; Folacci, M. ; Chassot, A. A. ; Fedou, S. ; Thézé, N. ; Zabelskii, D. ; Alekseev, A. ; Bamberg, E. ; Gordeliy, V.FZJ* ; Sandoz, G. (Corresponding author) ; Vivaudou, M. (Corresponding author)

2024
Nature Publishing Group UK [London]

This record in other databases:      

Please use a persistent id in citations: doi:10.1038/s41467-023-44548-6  doi:10.34734/FZJ-2024-03244

Abstract: Rhodopsins are ubiquitous light-driven membrane proteins with diverse functions, including ion transport. Widely distributed, they are also coded in the genomes of giant viruses infecting phytoplankton where their function is not settled. Here, we examine the properties of OLPVR1 (Organic Lake Phycodnavirus Rhodopsin) and two other type 1 viral channelrhodopsins (VCR1s), and demonstrate that VCR1s accumulate exclusively intracellularly, and, upon illumination, induce calcium release from intracellular IP3-dependent stores. In vivo, this light-induced calcium release is sufficient to remote control muscle contraction in VCR1-expressing tadpoles. VCR1s natively confer light-induced Ca2+ release, suggesting a distinct mechanism for reshaping the response to light of virus-infected algae. The ability of VCR1s to photorelease calcium without altering plasma membrane electrical properties marks them as potential precursors for optogenetics tools, with potential applications in basic research and medicine.

---

Contributing Institute(s):

1. Strukturbiochemie (IBI-7)

Research Program(s):

1. 5241 - Molecular Information Processing in Cellular Systems (POF4-524) (POF4-524)

Appears in the scientific report 2024

---

Database coverage:
; ; ; ; Article Processing Charges ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Agriculture, Biology and Environmental Sciences ; Current Contents - Life Sciences ; Current Contents - Physical, Chemical and Earth Sciences ; DOAJ Seal ; Essential Science Indicators ; Fees ; IF >= 15 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection ; Zoological Record

---