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@ARTICLE{Nimerovsky:1026616,
author = {Nimerovsky, Evgeny and Sieme, Daniel and Rezaie Ghaleh,
Nasrollah},
title = {{M}obility of sodium ions in agarose gels probed through
combined single- and triple-quantum {NMR}},
journal = {Methods},
volume = {228},
issn = {1046-2023},
address = {Orlando, Fla.},
publisher = {Academic Press},
reportid = {FZJ-2024-03470},
pages = {55-64},
year = {2024},
abstract = {Metal ions, including biologically prevalent sodium ions,
can modulate electrostatic interactions frequently involved
in the stability of condensed compartments in cells.
Quantitative characterization of heterogeneous ion dynamics
inside biomolecular condensates demands new experimental
approaches. Here we develop a 23Na NMR relaxation-based
integrative approach to probe dynamics of sodium ions inside
agarose gels as a model system. We exploit the electric
quadrupole moment of spin-3/2 23Na nuclei and, through
combination of single-quantum and triple-quantum-filtered
23Na NMR relaxation methods, disentangle the relaxation
contribution of different populations of sodium ions inside
gels. Three populations of sodium ions are identified: a
population with biexponential relaxation representing ions
within the slow motion regime and wo populations with
monoexponential relaxation but at different rates. Our study
demonstrates the dynamical heterogeneity of sodium ions
inside agarose gels and presents a new experimental approach
for monitoring dynamics of sodium and other spin-3/2 ions
(e.g. chloride) in condensed environments.},
cin = {IBI-7},
ddc = {540},
cid = {I:(DE-Juel1)IBI-7-20200312},
pnm = {5241 - Molecular Information Processing in Cellular Systems
(POF4-524)},
pid = {G:(DE-HGF)POF4-5241},
typ = {PUB:(DE-HGF)16},
pubmed = {38782295},
UT = {WOS:001265960700001},
doi = {10.1016/j.ymeth.2024.05.015},
url = {https://juser.fz-juelich.de/record/1026616},
}