TY  - JOUR
AU  - Küçükoğlu, Berk
AU  - Mohammed, Inayathulla
AU  - Guerrero-Ferreira, Ricardo C.
AU  - Ribet, Stephanie M.
AU  - Varnavides, Georgios
AU  - Leidl, Max Leo
AU  - Lau, Kelvin
AU  - Nazarov, Sergey
AU  - Myasnikov, Alexander
AU  - Kube, Massimo
AU  - Radecke, Julika
AU  - Sachse, Carsten
AU  - Müller-Caspary, Knut
AU  - Ophus, Colin
AU  - Stahlberg, Henning
TI  - Low-dose cryo-electron ptychography of proteins at sub-nanometer resolution
JO  - Nature Communications
VL  - 15
IS  - 1
SN  - 2041-1723
CY  - [London]
PB  - Nature Publishing Group UK
M1  - FZJ-2024-05612
SP  - 8062
PY  - 2024
AB  - Cryo-transmission electron microscopy (cryo-EM) of frozen hydrated specimens is an efficient method for the structural analysis of purified biological molecules. However, cryo-EM and cryo-electron tomography are limited by the low signal-to-noise ratio (SNR) of recorded images, making detection of smaller particles challenging. For dose-resilient samples often studied in the physical sciences, electron ptychography – a coherent diffractive imaging technique using 4D scanning transmission electron microscopy (4D-STEM) – has recently demonstrated excellent SNR and resolution down to tens of picometers for thin specimens imaged at room temperature. Here we apply 4D-STEM and ptychographic data analysis to frozen hydrated proteins, reaching sub-nanometer resolution 3D reconstructions. We employ low-dose cryo-EM with an aberration-corrected, convergent electron beam to collect 4D-STEM data for our reconstructions. The high frame rate of the electron detector allows us to record large datasets of electron diffraction patterns with substantial overlaps between the interaction volumes of adjacent scan positions, from which the scattering potentials of the samples are iteratively reconstructed. The reconstructed micrographs show strong SNR enabling the reconstruction of the structure of apoferritin protein at up to 5.8 Å resolution. We also show structural analysis of the Phi92 capsid and sheath, tobacco mosaic virus, and bacteriorhodopsin at slightly lower resolutions.
LB  - PUB:(DE-HGF)16
C6  - 39277607
UR  - <Go to ISI:>//WOS:001457649000008
DO  - DOI:10.1038/s41467-024-52403-5
UR  - https://juser.fz-juelich.de/record/1031216
ER  -