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@ARTICLE{Henderikx:1032201,
      author       = {Henderikx, Rene J. M. and Schotman, Maaike J. G. and
                      Shahzad, Saba and Fromm, Simon A. and Mann, Daniel and
                      Hennies, Julian and Heidler, Thomas V. and Ashtiani, Dariush
                      and Hagen, Wim J. H. and Jeurissen, Roger J. M. and Mattei,
                      Simone and Peters, Peter J. and Sachse, Carsten and Beulen,
                      Bart W. A. M. M.},
      title        = {{I}ce thickness control and measurement in the {V}itro{J}et
                      for time-efficient single particle structure determination},
      journal      = {Journal of structural biology},
      volume       = {216},
      number       = {4},
      issn         = {1047-8477},
      address      = {San Diego, Calif.},
      publisher    = {Elsevier},
      reportid     = {FZJ-2024-06070},
      pages        = {108139 -},
      year         = {2024},
      abstract     = {Embedding biomolecules in vitreous ice of optimal thickness
                      is critical for structure determination by
                      cryoelectronmicroscopy. Ice thickness assessment and
                      selection of suitable holes for data collection are
                      currentlypart of time-consuming preparatory routines
                      performed on expensive electron microscopes. To address
                      thischallenge, a routine has been developed to measure ice
                      thickness during sample preparation using an opticalcamera
                      integrated in the VitroJet. This method allows to estimate
                      the ice thickness with an error below ±20 nmfor ice layers
                      in the range of 0–70 nm. Additionally, we characterized
                      the influence of pin printing parametersand found that the
                      median ice thickness can be reproduced with a standard
                      deviation below ±11 nm forthicknesses up to 75 nm.
                      Therefore, the ice thickness of buffer-suspended holes on an
                      EM grid can be tuned andmeasured within the working range
                      relevant for single particle cryo-EM. Single particle
                      structures of apoferritinwere determined at two distinct
                      thicknesses of 30 nm and 70 nm. These reconstructions
                      demonstrate theimportance of ice thickness for
                      time-efficient cryo-EM structure determination.},
      cin          = {ER-C-3},
      ddc          = {540},
      cid          = {I:(DE-Juel1)ER-C-3-20170113},
      pnm          = {5352 - Understanding the Functionality of Soft Matter and
                      Biomolecular Systems (POF4-535) / 5241 - Molecular
                      Information Processing in Cellular Systems (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5352 / G:(DE-HGF)POF4-5241},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {39433138},
      UT           = {WOS:001343861100001},
      doi          = {10.1016/j.jsb.2024.108139},
      url          = {https://juser.fz-juelich.de/record/1032201},
}