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| Home > Publications database > Awake PET with the adenosine A1 receptor radiotracer F-18-CPFPX in rats: Optimization of reference region and route of injection |
| Home > Publications database > Awake PET with the adenosine A1 receptor radiotracer F-18-CPFPX in rats: Optimization of reference region and route of injection |
| Poster (After Call) | FZJ-2025-01029 |
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2024
Abstract: For the adenosine A1 receptor tracer F-18-CPFPX the olfactory bulb is an established reference region in rats. However, quantitative awake PET with radioactive point sources attached to the animal’s head for motion correction might be biased by spill-in from the nasal point source to the olfactory bulb. Moreover, for imaging without any anesthesia, intravenous (i.v.) injection of the tracer is demanding and stressful for the animal. This study investigates the feasibility of the pons as reference region and intraperitoneal (i.p.) tracer injection for quantitative analysis of F-18-CPFPX PET in awake rats. Eight male rats underwent six dynamic PET scans each following either an i.v. or i.p. bolus of F-18-CPFPX. For both conditions, two subsequent scans were performed under awake condition followed by a scan under anesthesia. The outcome parameter BPND determined via the simplified reference-tissue model (reference: olfactory bulb or pons) was evaluated in terms of variability and reproducibility. In-vitro H-3-DPCPX saturation autoradiography of the same animals served for validation of in-vivo outcome parameters.In-vitro Bmax and F-18-CPFPX BPNDpons correlated significantly (all p<0.01) for i.v. (r=0.81-0.86) and i.p. (r=0.81-0.88) injection route for awake and anesthetized condition. Test-retest stability of BPNDpons after i.p. tracer injection performed best when comparing the different conditions and gave reliable results in awake animals with high test-retest correlations (r=0.99, p<0.01) and an acceptable absolute variability (mean over all regions 15.3±5.8%).Quantitative awake small-animal PET imaging with F-18-CPFPX is improved by applying a reference region with lower probability of radioactive spill-in from point sources and practicability can be enhanced by i.p. tracer injection without loss of quantitative accuracy.
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