% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@INPROCEEDINGS{Kroll:1037886,
      author       = {Kroll, Tina and Miranda, Alan and Drechsel, Alexandra and
                      Klein, Sabina and Beer, Simone and Neumaier, Bernd and
                      Drzezga, Alexander and Verhaeghe, Jeroen and Elmenhorst,
                      David and Bauer, Andreas},
      title        = {{A}wake {PET} with the adenosine {A}1 receptor radiotracer
                      {F}-18-{CPFPX} in rats: {O}ptimization of reference region
                      and route of injection},
      reportid     = {FZJ-2025-01029},
      year         = {2024},
      abstract     = {For the adenosine A1 receptor tracer F-18-CPFPX the
                      olfactory bulb is an established reference region in rats.
                      However, quantitative awake PET with radioactive point
                      sources attached to the animal’s head for motion
                      correction might be biased by spill-in from the nasal point
                      source to the olfactory bulb. Moreover, for imaging without
                      any anesthesia, intravenous (i.v.) injection of the tracer
                      is demanding and stressful for the animal. This study
                      investigates the feasibility of the pons as reference region
                      and intraperitoneal (i.p.) tracer injection for quantitative
                      analysis of F-18-CPFPX PET in awake rats. Eight male rats
                      underwent six dynamic PET scans each following either an
                      i.v. or i.p. bolus of F-18-CPFPX. For both conditions, two
                      subsequent scans were performed under awake condition
                      followed by a scan under anesthesia. The outcome parameter
                      BPND determined via the simplified reference-tissue model
                      (reference: olfactory bulb or pons) was evaluated in terms
                      of variability and reproducibility. In-vitro H-3-DPCPX
                      saturation autoradiography of the same animals served for
                      validation of in-vivo outcome parameters.In-vitro Bmax and
                      F-18-CPFPX BPNDpons correlated significantly (all p<0.01)
                      for i.v. (r=0.81-0.86) and i.p. (r=0.81-0.88) injection
                      route for awake and anesthetized condition. Test-retest
                      stability of BPNDpons after i.p. tracer injection performed
                      best when comparing the different conditions and gave
                      reliable results in awake animals with high test-retest
                      correlations (r=0.99, p<0.01) and an acceptable absolute
                      variability (mean over all regions
                      $15.3±5.8\%).Quantitative$ awake small-animal PET imaging
                      with F-18-CPFPX is improved by applying a reference region
                      with lower probability of radioactive spill-in from point
                      sources and practicability can be enhanced by i.p. tracer
                      injection without loss of quantitative accuracy.},
      month         = {Apr},
      date          = {2024-04-10},
      organization  = {62. Jahrestagung der Deutschen
                       Gesellschaft für Nuklearmedizin,
                       Leipzig (Germany), 10 Apr 2024 - 13 Apr
                       2024},
      subtyp        = {After Call},
      cin          = {INM-2},
      cid          = {I:(DE-Juel1)INM-2-20090406},
      pnm          = {5253 - Neuroimaging (POF4-525) / NEURON Cofund - ERA NET
                      NEURON in the area of brain-related diseases and disorders
                      of the nervous system (680966)},
      pid          = {G:(DE-HGF)POF4-5253 / G:(EU-Grant)680966},
      typ          = {PUB:(DE-HGF)24},
      url          = {https://juser.fz-juelich.de/record/1037886},
}