% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Lu:1049765,
      author       = {Lu, Han and Garg, Shreyash and Lenz, Maximilian and
                      Vlachos, Andreas},
      title        = {{R}epetitive magnetic stimulation with i{TBS}600 induces
                      persistent structuraland functional plasticity in mouse
                      organotypic slice cultures},
      journal      = {Brain stimulation},
      volume       = {18},
      number       = {5},
      issn         = {1935-861X},
      address      = {New York, NY [u.a.]},
      publisher    = {Elsevier},
      reportid     = {FZJ-2025-05549},
      pages        = {1392-1402},
      year         = {2025},
      abstract     = {Background:Repetitive transcranial magnetic stimulation
                      (rTMS) is well known for its ability to induce synaptic
                      plasticity, yet its impact on structural and functional
                      remodeling within stimulated networks remains unclear. This
                      study investigates the cellular and network-level mechanisms
                      of rTMS-induced plasticity using a clinically approved
                      600-pulse intermittent theta burst stimulation (iTBS600)
                      protocol applied to mouse organotypic brain tissue
                      cultures.<br><br>Methods:We applied iTBS600 to
                      entorhino-hippocampal organotypic tissue cultures and
                      conducted a 24-hour analysis using c-Fos immunostaining,
                      whole-cell patch-clamp recordings, time-lapse imaging of
                      dendritic spines, and calcium imaging.<br><br>Results:We
                      observed long-term potentiation (LTP) of excitatory synapses
                      in dentate granule cells, characterized by increased mEPSC
                      frequencies and spine remodeling over time. c-Fos expression
                      in the dentate gyrus was transient and exhibited a clear
                      sensitivity to the orientation of the induced electric
                      field, suggesting a direction-dependent induction of
                      plasticity. Structural remodeling of dendritic spines was
                      temporally linked to enhanced synaptic strength, while
                      spontaneous calcium activity remained stable during the
                      early phase in the dentate gyrus, indicating the engagement
                      of homeostatic mechanisms. Despite the widespread electric
                      field generated by rTMS, its effects were spatially and
                      temporally precise, driving Hebbian plasticity and
                      region-specific spine dynamics.<br><br>Conclusions:These
                      findings provide mechanistic insights into how rTMS-induced
                      LTP promotes targeted plasticity while preserving network
                      stability. Understanding these interactions may help refine
                      stimulation protocols to optimize therapeutic outcomes.},
      cin          = {JSC},
      ddc          = {610},
      cid          = {I:(DE-Juel1)JSC-20090406},
      pnm          = {5111 - Domain-Specific Simulation $\&$ Data Life Cycle Labs
                      (SDLs) and Research Groups (POF4-511) / SLNS - SimLab
                      Neuroscience (Helmholtz-SLNS) / JL SMHB - Joint Lab
                      Supercomputing and Modeling for the Human Brain (JL
                      SMHB-2021-2027)},
      pid          = {G:(DE-HGF)POF4-5111 / G:(DE-Juel1)Helmholtz-SLNS /
                      G:(DE-Juel1)JL SMHB-2021-2027},
      typ          = {PUB:(DE-HGF)16},
      doi          = {10.1016/j.brs.2025.07.008},
      url          = {https://juser.fz-juelich.de/record/1049765},
}