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| Hauptseite > Online First > How To Make Your Cell Tracker Say "I dunno!" |
| Hauptseite > Online First > How To Make Your Cell Tracker Say "I dunno!" |
| Contribution to a conference proceedings/Contribution to a book | FZJ-2025-05660 |
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2025
Abstract: Cell tracking is a key computational task in live-cell microscopy, but fully automated analysis of high-throughput imaging requires reliable and, thus, uncertainty-aware data analysis tools, as the amount of data recorded within a single experiment exceeds what humans are able to overlook. We here propose and benchmark various methods to reason about and quantify uncertainty in linear assignment-based cell tracking algorithms. Our methods take inspiration from statistics and machine learning, leveraging two perspectives on the cell tracking problem explored throughout this work: Considering it as a Bayesian inference problem and as a classification problem. Our methods admit a framework-like character in that they equip any frame-to-frame tracking method with uncertainty quantification. We demonstrate this by applying it to various existing tracking algorithms including the recently presented Transformer-based trackers. We demonstrate empirically that our methods yield useful and well-calibrated tracking uncertainties.
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