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| Home > Publications database > Alpha-Amylase from germinating soybean (Glycine max) seeds - Purification, characterization and sequential similarity of conserved and catalytic amino acid residues |
| Home > Publications database > Alpha-Amylase from germinating soybean (Glycine max) seeds - Purification, characterization and sequential similarity of conserved and catalytic amino acid residues |
| Journal Article | PreJuSER-10928 |
; ; ; ;
2010
Elsevier Science
New York, NY [u.a.]
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Please use a persistent id in citations: doi:10.1016/j.phytochem.2010.06.012
Abstract: Starch hydrolyzing amylase from germinated soybeans seeds (Glycine max) has been purified 400-fold to electrophoretic homogeneity with a final specific activity of 384 units/mg. SDS-PAGE of the final preparation revealed a single protein band of 100 kDa, whereas molecular mass was determined to be 84 kDa by MALDI-TOF and gel filtration on Superdex-200 (FPLC). The enzyme exhibited maximum activity at pH 5.5 and a pI value of 4.85. The energy of activation was determined to be 6.09 kcal/mol in the temperature range 25-85 degrees C. Apparent Michaelis constant (K(m)((app))) for starch was 0.71 mg/mL and turnover number (k(cat)) was 280 s(-1) in 50 mM sodium acetate buffer, pH 5.5. Thermal inactivation studies at 85 degrees C showed first-order kinetics with rate constant (k) equal to 0.0063 min(-1). Soybean alpha-amylase showed high specificity for its primary substrate starch. High similarity of soybean alpha-amylase with known amylases suggests that this alpha-amylase belongs to glycosyl hydrolase family 13. Cereal alpha-amylases have gained importance due to their compatibility for biotechnological applications. Wide availability and easy purification protocol make soybean as an attractive alternative for plant alpha-amylase. Soybean can be used as commercially viable source of alpha-amylase for various industrial applications.
Keyword(s): Amino Acid Sequence (MeSH) ; Amino Acids: analysis (MeSH) ; Germination: physiology (MeSH) ; Hydrogen-Ion Concentration (MeSH) ; Plants: enzymology (MeSH) ; Seeds: enzymology (MeSH) ; Seeds: metabolism (MeSH) ; Sequence Homology, Amino Acid (MeSH) ; Soybeans: enzymology (MeSH) ; Soybeans: metabolism (MeSH) ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (MeSH) ; alpha-Amylases: metabolism (MeSH) ; Amino Acids ; alpha-Amylases ; J ; alpha-Amylase (auto) ; Purification (auto) ; Glycine max (auto) ; MALDI-TOF (auto) ; Sequence homology (auto)
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