Journal Article

The cellular uptake and intracellular distribution of fluorescently labeled ultrasmall gold nanoparticles (∼2 nm) inhuman RPE-1 cells were investigated by a complementary combination of confocal laser scanning microscopy (CLSM), structuredillumination microscopy (SIM), and cryo-electron microscopy (cryo-EM). The nanoparticles were synthesized by a modified Brust–Schiffrin route, functionalized with glutathione, and covalently labeled with AlexaFluor-647, enabling fluorescence-based tracking.High-resolution transmission electron microscopy showed a narrow particle size distribution with an average core diameter of 2.3 nm.Live-cell CLSM and SIM imaging revealed intracellular nanoparticle-associated fluorescence and pronounced colocalization withlysosomes, consistent with accumulation along the endolysosomal pathway [...]

The parent-child relationship is crucial for children's socio-emotional development. Previous research, mostly in mothers of infants, has implicated neural networks subserving reward, saliency, and theory of mind (ToM). [...]