%0 Journal Article
%A Oszlanczi, A.
%A Bóta, A.
%A Klumpp, E.
%T Influence of aminoglycoside antibiotics on the thermal behaviour and structural features of DPPE - DPPG model membranes
%J Colloids and surfaces / B
%V 75
%@ 0927-7765
%C Amsterdam [u.a.]
%I Elsevier Science
%M PreJuSER-11680
%P 141 - 148
%D 2010
%Z This work was supported by the Hungarian Scientific Funds OTKA (Bota, T 43055) and a German-Hungarian Research Project. We wish to thank Cs Novak for giving the opportunity for the calorimetric measurements, and Mrs. T. Kiss and Mrs. E Toth for technical assistance at the freeze-fracture and DSC measurements, respectively.
%X The effects of three aminoglycosides (AGs), tobramycin, streptomycin and spectinomycin on 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE)-1,2-dipalmitoyl-sn-glycero-3-[phospho-rac-1-glycerol] (DPPG)/water systems with 0.05 and 0.2 DPPG/DPPE+DPPG molar fractions were examined by using differential scanning calorimetry (DSC), simultaneous small- and wide-angle X-ray scattering (SWAXS), and freeze-fracture transmission electronmicroscopy (FF-TE) at 10(-1) AG/lipid molar ratio in the doped systems. The effects induced by the AGs strongly depend on DPPG/DPPE+DPPG molar fraction. In the presence of the AGs regular lyotropic structures form as opposed to the complex and badly correlated layer structures of the pure 0.2 DPPG/DPPE+DPPG molar fraction system. The investigated AGs possess different structural and thermotropic effects: tobramycin and streptomycin which are true aminoglycosides decrease the main transition enthalpy in both model systems and induce the formation of correlated layer arrangements, while the occurrence of spectinomycin, a non-aminoglycoside aminocyclitol still ranked as an AG, results in a hexagonal phase as it can be deduced from the SAXS patterns and visually observed in the electronmicrographs.
%K Aminoglycosides: chemistry
%K Aminoglycosides: pharmacology
%K Anti-Bacterial Agents: chemistry
%K Anti-Bacterial Agents: pharmacology
%K Calorimetry, Differential Scanning
%K Freeze Fracturing
%K Membranes, Artificial
%K Microscopy, Electron, Transmission
%K Models, Chemical
%K Phase Transition: drug effects
%K Phosphatidylethanolamines: chemistry
%K Phosphatidylglycerols: chemistry
%K Scattering, Small Angle
%K Spectinomycin: chemistry
%K Spectinomycin: pharmacology
%K Surface Properties: drug effects
%K Temperature
%K X-Ray Diffraction
%K Aminoglycosides (NLM Chemicals)
%K Anti-Bacterial Agents (NLM Chemicals)
%K Membranes, Artificial (NLM Chemicals)
%K Phosphatidylethanolamines (NLM Chemicals)
%K Phosphatidylglycerols (NLM Chemicals)
%K Spectinomycin (NLM Chemicals)
%K 1,2-dipalmitoyl-3-phosphatidylethanolamine (NLM Chemicals)
%K 1,2-dipalmitoylphosphatidylglycerol (NLM Chemicals)
%K J (WoSType)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:19748239
%U <Go to ISI:>//WOS:000278695300018
%R 10.1016/j.colsurfb.2009.08.027
%U https://juser.fz-juelich.de/record/11680