Journal Article

PreJuSER-11680

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Influence of aminoglycoside antibiotics on the thermal behaviour and structural features of DPPE - DPPG model membranes

Oszlanczi, A. ; Bóta, A. ; Klumpp, E.FZJ*

2010
Elsevier Science Amsterdam [u.a.]

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Please use a persistent id in citations: doi:10.1016/j.colsurfb.2009.08.027

Abstract: The effects of three aminoglycosides (AGs), tobramycin, streptomycin and spectinomycin on 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE)-1,2-dipalmitoyl-sn-glycero-3-[phospho-rac-1-glycerol] (DPPG)/water systems with 0.05 and 0.2 DPPG/DPPE+DPPG molar fractions were examined by using differential scanning calorimetry (DSC), simultaneous small- and wide-angle X-ray scattering (SWAXS), and freeze-fracture transmission electronmicroscopy (FF-TE) at 10(-1) AG/lipid molar ratio in the doped systems. The effects induced by the AGs strongly depend on DPPG/DPPE+DPPG molar fraction. In the presence of the AGs regular lyotropic structures form as opposed to the complex and badly correlated layer structures of the pure 0.2 DPPG/DPPE+DPPG molar fraction system. The investigated AGs possess different structural and thermotropic effects: tobramycin and streptomycin which are true aminoglycosides decrease the main transition enthalpy in both model systems and induce the formation of correlated layer arrangements, while the occurrence of spectinomycin, a non-aminoglycoside aminocyclitol still ranked as an AG, results in a hexagonal phase as it can be deduced from the SAXS patterns and visually observed in the electronmicrographs.

Keyword(s): Aminoglycosides: chemistry (MeSH) ; Aminoglycosides: pharmacology (MeSH) ; Anti-Bacterial Agents: chemistry (MeSH) ; Anti-Bacterial Agents: pharmacology (MeSH) ; Calorimetry, Differential Scanning (MeSH) ; Freeze Fracturing (MeSH) ; Membranes, Artificial (MeSH) ; Microscopy, Electron, Transmission (MeSH) ; Models, Chemical (MeSH) ; Phase Transition: drug effects (MeSH) ; Phosphatidylethanolamines: chemistry (MeSH) ; Phosphatidylglycerols: chemistry (MeSH) ; Scattering, Small Angle (MeSH) ; Spectinomycin: chemistry (MeSH) ; Spectinomycin: pharmacology (MeSH) ; Surface Properties: drug effects (MeSH) ; Temperature (MeSH) ; X-Ray Diffraction (MeSH) ; Aminoglycosides ; Anti-Bacterial Agents ; Membranes, Artificial ; Phosphatidylethanolamines ; Phosphatidylglycerols ; Spectinomycin ; 1,2-dipalmitoyl-3-phosphatidylethanolamine ; 1,2-dipalmitoylphosphatidylglycerol ; J ; Aminoglycosides (auto) ; DPPE-DPPG liposomes (auto) ; DSC (auto) ; Small- and wide-angle X-ray scattering (SWAXS) (auto) ; Freeze-fracture (auto)

Note: This work was supported by the Hungarian Scientific Funds OTKA (Bota, T 43055) and a German-Hungarian Research Project. We wish to thank Cs Novak for giving the opportunity for the calorimetric measurements, and Mrs. T. Kiss and Mrs. E Toth for technical assistance at the freeze-fracture and DSC measurements, respectively.

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Research Program(s):

1. Terrestrische Umwelt (P24)

Appears in the scientific report 2010

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