TY  - JOUR
AU  - Oszlanczi, A.
AU  - Bóta, A.
AU  - Klumpp, E.
TI  - Influence of aminoglycoside antibiotics on the thermal behaviour and structural features of DPPE - DPPG model membranes
JO  - Colloids and surfaces / B
VL  - 75
SN  - 0927-7765
CY  - Amsterdam [u.a.]
PB  - Elsevier Science
M1  - PreJuSER-11680
SP  - 141 - 148
PY  - 2010
N1  - This work was supported by the Hungarian Scientific Funds OTKA (Bota, T 43055) and a German-Hungarian Research Project. We wish to thank Cs Novak for giving the opportunity for the calorimetric measurements, and Mrs. T. Kiss and Mrs. E Toth for technical assistance at the freeze-fracture and DSC measurements, respectively.
AB  - The effects of three aminoglycosides (AGs), tobramycin, streptomycin and spectinomycin on 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE)-1,2-dipalmitoyl-sn-glycero-3-[phospho-rac-1-glycerol] (DPPG)/water systems with 0.05 and 0.2 DPPG/DPPE+DPPG molar fractions were examined by using differential scanning calorimetry (DSC), simultaneous small- and wide-angle X-ray scattering (SWAXS), and freeze-fracture transmission electronmicroscopy (FF-TE) at 10(-1) AG/lipid molar ratio in the doped systems. The effects induced by the AGs strongly depend on DPPG/DPPE+DPPG molar fraction. In the presence of the AGs regular lyotropic structures form as opposed to the complex and badly correlated layer structures of the pure 0.2 DPPG/DPPE+DPPG molar fraction system. The investigated AGs possess different structural and thermotropic effects: tobramycin and streptomycin which are true aminoglycosides decrease the main transition enthalpy in both model systems and induce the formation of correlated layer arrangements, while the occurrence of spectinomycin, a non-aminoglycoside aminocyclitol still ranked as an AG, results in a hexagonal phase as it can be deduced from the SAXS patterns and visually observed in the electronmicrographs.
KW  - Aminoglycosides: chemistry
KW  - Aminoglycosides: pharmacology
KW  - Anti-Bacterial Agents: chemistry
KW  - Anti-Bacterial Agents: pharmacology
KW  - Calorimetry, Differential Scanning
KW  - Freeze Fracturing
KW  - Membranes, Artificial
KW  - Microscopy, Electron, Transmission
KW  - Models, Chemical
KW  - Phase Transition: drug effects
KW  - Phosphatidylethanolamines: chemistry
KW  - Phosphatidylglycerols: chemistry
KW  - Scattering, Small Angle
KW  - Spectinomycin: chemistry
KW  - Spectinomycin: pharmacology
KW  - Surface Properties: drug effects
KW  - Temperature
KW  - X-Ray Diffraction
KW  - Aminoglycosides (NLM Chemicals)
KW  - Anti-Bacterial Agents (NLM Chemicals)
KW  - Membranes, Artificial (NLM Chemicals)
KW  - Phosphatidylethanolamines (NLM Chemicals)
KW  - Phosphatidylglycerols (NLM Chemicals)
KW  - Spectinomycin (NLM Chemicals)
KW  - 1,2-dipalmitoyl-3-phosphatidylethanolamine (NLM Chemicals)
KW  - 1,2-dipalmitoylphosphatidylglycerol (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:19748239
UR  - <Go to ISI:>//WOS:000278695300018
DO  - DOI:10.1016/j.colsurfb.2009.08.027
UR  - https://juser.fz-juelich.de/record/11680
ER  -