TY  - JOUR
AU  - Smith, A.-S.
AU  - Fenz, S.F.
AU  - Sengupta, K.
TI  - Inferring spatial organization of bonds within adhesion clusters by exploiting fluctuations of soft interfaces
JO  - epl
VL  - 89
SN  - 0302-072X
CY  - Les Ulis
PB  - EDP Sciences
M1  - PreJuSER-11684
SP  - 28003
PY  - 2010
N1  - We are grateful to S. GONNENWEIN for giving access to her data (figs. 1 and 4). We thank R. Merkel, E. SACKMANN, U. SEIFERT, C. MONZEL and L. LIMOZIN for stimulating discussions and R. Merkel for careful reading of the manuscript. A-SS was funded by DFG-SE 1119/2-1. KS and SFF thank R. MERKEL for support.
AB  - Detecting the organization of bonds within adhesion domains connecting two interacting membranes is, at present, extremely challenging. Herein we present a technique, based on Reflection Interference Contrast Microscopy, which uses spontaneous thermal fluctuations of a soft interface as a tool to identify the organization of specific ligand-receptor bonds. The key is a time-resolved analysis of micro-interferometric data that systematically quantifies fluctuations and enables the detection of their suppression due to the formation of bonds, which, in turn, allows the identification of the bond organization without the use of fluorescent labelling. The identification of a new type of bond organization characterized by sparsely distributed bonds, as well as detection of pinning centres of nanometric size is presented. Copyright (C) EPLA, 2010
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000274848500034
DO  - DOI:10.1209/0295-5075/89/28003
UR  - https://juser.fz-juelich.de/record/11684
ER  -